The final parts of the chapter are concerned with the uses and misuses of genetic language and the need for a better societal understanding of the benefits and limitations of genetic testing.Bourn, DavidNorthern Genetics Service
Enzymaticdigestionis an effective alternative to the mechanical shearing methods.Endonucleasesand nicking enzymes are usually employed to cleave both strands of DNA or nick individual strands to generate double-stranded breakage. To avoid ...
The 9-1-1 DNA checkpoint clamp is loaded onto 5′-recessed DNA to activate the DNA damage checkpoint that arrests the cell cycle. The 9-1-1 clamp is a heterotrimeric ring that is loaded in Saccharomyces cerevisiae by Rad24-RFC (hRAD17-RFC), an alternate clamp loader in which Rad24 rep...
Transcription-replication conflicts (TRCs), especially Head-On TRCs (HO-TRCs) can introduce R-loops and DNA damage, however, the underlying mechanisms are still largely unclear. We previously identified a chloroplast-localized RNase H1 protein AtRNH1C that can remove R-loops and relax HO-TRCs for...
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In this step, four rounds of DNA stands (the first strands) complementary to the bisulfite-converted DNA are synthesized using Klenow DNA polymerase and random primers with the adapter I being tagged. ReagentAmountFinal concentration Blue Buffer (10×) 2.5 μL 1× P5-N9-oligo1 (10 μΜ) ...
ConsensusCruncher is a tool that suppresses errors in next-generation sequencing data by using unique molecular identifiers (UMIs) to amalgamate reads derived from the same DNA template into a consensus sequence. - pughlab/ConsensusCruncher
The bottom strands of the duplexes were 5′-end-labeled. NoPt lanes, unplatinated duplex; Pt/CN − lanes, the duplex containing an interstrand CL with platinum removed by NaCN after modification by DMS. For other details, see “Results.” Chemical Probes of DNA Conformation Among the ...
In the present study, using the RNA deep sequencing data, we systematically survey a type of non-canonical fusions between the RNA transcripts from the two opposite DNA strands. We name the products of such fusion events cross-strand chimeric RNA (cscRNA). Hundreds to thousands of cscRNAs ...
et al. Long inverted repeat transiently stalls DNA replication by forming hairpin structures on both leading and lagging strands. Genes Cells 21, 136–145 (2016). Article CAS PubMed Google Scholar Yeeles, J. T., Deegan, T. D., Janska, A., Early, A. & Diffley, J. F. Regulated ...