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aThe enzymatic activity ofIsPETase-Ppwith or without deglycosylation. S: the supernatant of the high-density fermentation; D: dialyzed supernatant of the high-density fermentation; D + Endo H: dialyzing prior to deglycosylation with Endo H; Endo H + D: deglycosylation with Endo H pri...
The purity of these proteins was confirmed by single band staining on SDS-polyacrylamide gel electrophoresis (data not shown). Circular dichroism of these purified proteins was used to monitor the refolding process. Fig. 1 shows the proportional secondary structure of the truncated fragments derived ...
One firm, Browne Time Sharing Inc., specialized in online text editing and process- ing services.44 Browne launched its service in 1969, using an IBM 370 mainframe connected to dial-in telephone lines, and marketed to users needing to make frequent revisions to long doc- uments. Its main ...
The cells in the HK-2 group were irregular islands with cells covering the whole microscope field, precise connections, almost no gaps between cells, and a wide range of cells fused to grow into sheets or laminated growth (Fig. 4a). The cell morphology was irregular, with obvious atrophy in...
SCP Dial-Up Networking Script (Microsoft Corporation) SCP Atari Disk Image (Atari) SCP Script SCP SCAR Divi Macro Script (Fr閐閞ic Hannes) SCPR ScreenCorder Project File (MatchWare A/S) SCPT AppleScript Script (Apple Inc.) SCQ?? SAS Version 6 Sequential Access File Catalog (SAS Institute...
Crystals were obtained by dialyzing the concentrated protein against 20 mM MES (pH 6.5), 75 mM NaCl, 10 mM DTT, 1 mM EDTA, 10% (w/v) glycerol. In general, crystals in the form of hexagonal rods appeared overnight and continued to grow for several days, reaching a final size of 0....
The cells in the HK-2 group were irregular islands with cells covering the whole microscope field, precise connections, almost no gaps between cells, and a wide range of cells fused to grow into sheets or laminated growth (Fig. 4a). The cell morphology was irregular, with obvious atrophy in...
Perez et al., “Inhibition of brush border dipeptidase with cilastatin reduces toxic accumulation of cyclosporine A in kidney proximal tubule epithelial cells,” Nephrol Dial Transplant 19:2445-2455, 2004. Razani et al., Caveolae: From Cell Biology to Animal Physiology, Pharmacological Reviews 54(...