The development of tools that allow genetic manipulation is crucial for further strain improvement and the functional characterization of its genes. In this context, the CRISPR/Cas9 system represents an excellent option for genome editing due to its high efficiency and versatility. To establish CRISPR...
1.1. The discovery and development of the CRISPR/Cas9 system for genome editing in eukaryotes The genome of the adaptive bacterial immune system consists of CRISPR (clustered regularly interspaced short palindromic repeats) arrays that consist of direct repeat DNA sequences (Wiedenheft et al., 2012...
Evolving techniques of targeted genome editing, specifically the CRISPR/Cas9 system, allow for the generation of cell lines bearing gene-specific knock-outs, knock-in reporters, and precise mutations. However, there are increasing concerns related to the transfection efficiency, cell viability, and ...
该研究建立了可用于mtDNA的CRISPR/Cas9编辑体系mito-Cas9,并通过无PCR扩增的三代测序技术,直接验证到该体系编辑后的mtDNA分子,解决了一直以来围绕“CRISPR/Cas9体系是否可以编辑mtDNA”这一问题的争议。虽然目前mito-Cas9体系对mtDNA的编辑效率还很低,离临床基因治疗预期差距甚远,但基于CRISPR/Cas9对比其他基因编辑技术的...
CRISPR/Cas9 has become the system of choice. CRISPR/Cas9 is poised to transform biological research and the development of therapeutics for debilitating human conditions and diseases. In this review, we provide a brief background of genome-engineering platforms, with a special emphasis on the CRISPR...
Integration of high-risk HPV genomes into cellular chromatin has been confirmed to promote cervical carcinogenesis, with HPV16 being the most prevalent high-risk type. Herein, we evaluated the therapeutic effect of the CRISPR/Cas9 system in cervical carc
using the CRISPR/Cas9 system received: 10 November 2015 accepted: 07 March 2016 Published: 23 March 2016 Yao Zu1,*, Xushuai Zhang1,*, Jianfeng Ren1, Xuehong Dong1, Zhe Zhu1, Liang Jia1, Qinghua Zhang1 & Weiming Li1,2 Lampreys are extant representatives of agnathans...
The establishment of left-right body asymmetry is a key biological process that is tightly regulated genetically. In the first application of CRISPR/Cas9 to a mollusc, we show decisively that the actin-related diaphanous gene Lsdia1 is the single maternal gene that determines the shell coiling di...
Photoactivatable CRISPR-Cas9 for optogenetic genome editing. Nature Biotechnology. 6月15日,发表在《Nature Biotechnology》上的一项研究中,科学家们结合CRISPR与光遗传学构建出了一种系统:一种光激活的新型Cas9核酸酶使得研究人员能够在空间和时间上更好地控制RNA引导的核酸酶的活性。
The timeline of the CRISPR/Cas9 system. After over four decades of development since the discovery of CRISPR/Cas9 in prokaryotes, CASGEVY™, the first gene therapy protocol approved by the FDA, has evolved into an indispensable tool for disease research and treatment. In 1987, the initial dis...