In the present study, we report the de novo microbial biosynthesis of jasmonic acid and its derivatives, methyl jasmonate and jasmonoyl isoleucine, from glucose using an engineered baker’s yeast. The study uses enzymes located in the endoplasmic reticulum and cytosol to generate the intermediates ...
Discussion Although ubiquitin was discovered 40 years ago, the mechanism of its de novo synthesis has not received much attention. Earlier studies led to the idea that ubiquitin precursors might be processed co-translationally. The evidence supporting this idea was of two types. First, it was ...
Biomedical & Environmental Mass SpectrometryJanssens JJ, Schepens PJC (1988) Biomed. Environ. Mass Spectrom. 16: 179Janssens, J.J.; Schepens, P.J.C. (1988): On the de novo Synthesis of Polychlorinated Dibenzo-p-dioxins and Dibenzofurans; Biomed. Env. Mass. Spec.; 16: 179-182...
In this study, we show that the purified NS5 alone is sufficient for the synthesis of the two products and that the template-length RNA is the product of de novo initiation. Furthermore, the incubation temperature during initiation, but not elongation phase of RNA synthesis modulates the relati...
Expiants of mature dormant tubers of Helianthus tuberosus were grown in the absence of carbon source with different auxins. After transfer to a glucose medium, the explants were able to initiate a de novo synthesis of fructosans. This synthesis was affected by auxins, kinetin, and gibberellic ...
Previous screening of a transposon mutant library for replication within HeLa cells has suggested that nadB, encoding a putative l-aspartate oxidase required for de novo NAD synthesis, is needed for intracellular replication. Here, using genetic complementation of two independent nadB mutants and ...
The first strand of cDNA was synthesised using random hexamer primers, followed by synthesis of the second strand. The short fragments were purified with the QIAquick PCR Purification kit (Qiagen, Valencia, CA) for both end repair and the poly (A) addition reaction. The purified DNA libraries...
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Involvement of DED proteins in the inflammasome. (a) Full activation of the inflammasomes requires a two-step process. Firstly, a priming signal is induced by a PAMP (such as LPS) activating a transcriptional cascade resulting in thede novosynthesis of inflammasome components such as NLRP3 and ...
De novo Assembly of the Indo-Pacific Humpback Dolphin Leucocyte Transcriptome to Identify Putative Genes Involved in the Aquatic Adaptation and Immune Response. PLoS ONE 8, e72417, doi: 10.1371/journal.pone.0072417 (2013). Article CAS ADS Google Scholar Dobrynin, P. et al. Genomic legacy of ...