In the present study, we report the de novo microbial biosynthesis of jasmonic acid and its derivatives, methyl jasmonate and jasmonoyl isoleucine, from glucose using an engineered baker’s yeast. The study uses enzymes located in the endoplasmic reticulum and cytosol to generate the intermediates ...
Nakagawa, Y., Rustow, B., Rabe, H., Kunze, D., Waku, K., The de novo synthesis of molecular species of phosphatidylinositol from endogenously labeled CDP diacylglycerol in alveolar macrophage microsomes. Arch Biochern Biophys, 268(2):559-566, 1989....
brucei homologues of de novo GDP-fucose biosynthesis enzymes and demonstrated their ability to convert GDP-mannose to GDP-fucose in vitro. Both the incubation of GDP-mannose with TbGMD alone in vitro (supplemented with NADPH) and overexpression of TbGMD in vivo resulted in the appearance of a ...
Involvement of DED proteins in the inflammasome. (a) Full activation of the inflammasomes requires a two-step process. Firstly, a priming signal is induced by a PAMP (such as LPS) activating a transcriptional cascade resulting in thede novosynthesis of inflammasome components such as NLRP3 and ...
enabled de novo synthesis of DMX and increased DMX production by enhancing PTase expression and NC supply.dOptimizing the MVA pathway and overexpressingIDI1-HlPT1LΔ1-86perincreased DMX production. All strains were cultivated in 100 mL shake flasks containing 20 mL of minimal medium. Mean ...
Using mass spectrometry we found that lysophospholipid levels decreased drastically from G2/M to G1 phase, while de novo phosphatidylcholine synthesis, the main phospholipid in mammalian cells, increased, suggesting that enhanced membrane production was concomitant to a decrease in its turnover. In ...
Macrophage de novo NAD(+) synthesis specifies immune function in aging and inflammation. Nat Immunol. 2019;20:50–63. Article CAS PubMed Google Scholar Verdin E. NAD+ in aging, metabolism, and neurodegeneration. Science. 2015;350:1208–13. Article CAS PubMed Google Scholar Cantó C, Hout...
The modern evolutionary synthesis93postulates that evolution acts mainly via natural selection on phenotypes, ultimately affecting DNA sequences. The discovery that non-DNA sequence information, such as parental, ecological, behavioural and cultural information, can be heritable94has not broken the modern...
To explain this phenomenon, we first briefly introduce the synthesis process of PC. Two pathways are responsible for the de novo synthesis of PC, namely the phosphatidylethanolamine-N-methyltransferase (PEMT) and the Kennedy pathways. PEMT performs three sequential methylation reactions to convert ...
Hepatic de novo lipogenesis (DNL) is the biochemical process of synthesising fatty acids from acetyl-CoA subunits that are produced from a number of different pathways within the cell, most commonly carbohydrate catabolism. In addition to glucose which most commonly supplies carbon units for DNL, ...