SARS-CoV-2allosterycryo-EMfurin cleavagespikeThe SARS-CoV-2 spike (S) protein is the target of vaccine design efforts to end the COVID-19 pandemic. Despite a low mutation rate, isolates with the D614G substitution in the S protein appeared early during the pandemic, and are now the ...
The D614G mutation in the spike protein of SARS-CoV-2 alters the fitness of the virus, leading to the dominant form observed in the COVID-19 pandemic. However, the molecular basis of the mechanism by which this mutation enhances fitness is not clear. Here we demonstrated by cryo-electron ...
CoV-2 BA.1 spike as target cells.hADCP activity of mAbs with HEK293T cells expressing SARS-CoV-2 BA.1 spike as target cells. Irrelevant mAb with the same constant region was used as an isotype. Source data are provided as a Source Data file. Full size image ADCC and ADCP activity Th...
SARS (Hong Kong strain), and SARS-CoV-2 ancestral and delta strains. Infection and replication of these sarbecoviruses were compared in stem cell–derived neural precursor cells (NPCs
Mutations are known to play a critical role in how a species evolves.In a recent article that appeared in the journal Cell,Yurkovetskiy et al.1 reported the structural and functional properties of the SARS-CoV-2 spike protein variant D614G that explains the basis of its increased infectivity...
The SARS-CoV-2 spike protein interactswith ACE2 to facilitate virus entry into the host cell.2,3 Yurkovetskiyet al. characterized one of the single-nucleotide polymorphismsA23403G, out of the more than 12,000 reported in GISAID, whichencodes the spike protein variant D614G. The frequency ...
number of mutations were identified across the SARS-CoV-2 genome,including the region encoding S glycoprotein.Nevertheless,the functional implications of these mutations remain largely unknown,we here reported the mutation analysis of S gene and a functional exploration of the dominant D614G mutation.Xi...
Bimodular effects of D614G mutation on the spike glycoprotein of SARS-CoV-2 enhance protein processing, membrane fusion, and viral infectivitydoi:10.1038/s41392-020-00392-4Xiaoyi JiangZhengrong ZhangChenxi WangHongguang RenQiang SunSignal Transduction and Targeted Therapy...
Tracking changes in SARS-CoV-2 Spike: evidence that D614G increases infectivity of the COVID-19 virusdoi:10.1016/j.cell.2020.06.043Bette KorberWill FischerSandrasegaram GnanakaranMatthew Wyles
SUMMARYThe SARS-CoV-2 spike (S) protein variant D614G supplanted the ancestral virus worldwide in a matter of months. Here we show that D614G was more infectious than the ancestral form on human lung cells, colon cells, and cells rendered permissive by ectopic expression of various mammalian...