Chimeric antigen receptor (CAR) T cell therapy involves the genetic modification of T cells to recognize and bind to specific cancer cell surface antigens, thereby enabling them to effectively target and eliminate cancer cells [1]. Since the approval of the first CAR-T cell product targeting the...
Advice is also given on complying with current legal and ethical requirements when deriving cell lines from human and animal tissues, the selection and maintenance of equipment and how to deal with problems that may arise. INTRODUCTION Problems associated with cell culture, such as cell line ...
Robust characterization of the protein corona—the layer of proteins that spontaneously forms on the surface of nanoparticles immersed in biological fluids—is vital for prediction of the safety, biodistribution, and diagnostic/therapeutic efficacy of na
Biopharmaceuticals have revolutionized the field of medicine in the types of active ingredient molecules and treatable indications. Adoption of Quality by
Additionally, the article will analyze the current state of mRNA vaccine technology and highlight future directions for the development and implementation of this promising vaccine platform as a mainstream therapeutic option. The review will also discuss potential challenges and limitations of mRNA ...
Enrichment of ADP-ribosylated Peptides from HeLa Cell Lysate To induce PAR formation, cells were treated with various concentrations of H2O2 for 10 min and lysed in lysis buffer (50 mm Tris pH 8, 1% Nonidet P-40, 400 mm NaCl, 1 mm EDTA, 0.1% sodium deoxycholate, complete protease inhibi...
et al. (1992) showed how the application of a static magnetic field of 1.5 T for 96 h on “immortal” human cervical cancer cells (HeLa) did not produce significant changes in cell shape [18]. Such results are in contrast with those obtained from Pacini S. et al. (1999), who ...
Proportion of the HeLa cell genome complementary to the transfer RNA and 5S RNA. J. Mol. Biol. 56, 535–553 (1971). Article CAS PubMed Google Scholar Sprinzl, M., Horn, C., Brown, M., Ioudovitch, A. & Steinberg, S. Compilation of tRNA sequences and sequences of tRNA genes....
In this Review, we summarize the relationship between the metabolic and molecular pathways of iron signalling and ferroptosis in the context of cardiovascular disease. We also discuss the potential targets of ferroptosis in the treatment of cardiovascular disease and describe the current limitations and ...
The current detection technology of H2S concentration in tissues and cells is still immature. Introduction There are currently three well-known gasotransmitters: carbon monoxide (CO), nitric oxide (NO), and hydrogen sulfide (H2S). Endogenous H2S as the third gaseous signaling molecule is produced th...