In all TLC lanes, the radioactivity associated with each band was quantified, and the counts were normalized to the OD600 at the time of harvest to correct for variations in bacterial mass across samples. For unlabeled lipid analysis, equivalent weights of total lipid fraction from each strain ...
Finally, phosphopeptides were eluted with 20 μL of 1% NH4OH buffer (pH ∼11), acidified with 2 μl of formic acid (pH 3) and dried down for MS analysis. LC-MS/NS analysis Nanoflow LC-MS/MS was performed by coupling a Dionex 3000 (Thermo Fisher Scientific) to a QExactive Orbitrap...
L21 dry cell weight3, it is also capable of performing posttranslational modifications, including the formation of correct disulfide bridges and the glycosylation of secretory proteins, rendering P. pastoris specifically suitable for the production of complex eukaryotic proteins4. Glycosylation has long ...
To get the corrected centrosome intensity measurement the following equation was applied: Scorrect ¼ (S À B) Â B À 1. For the measurement of centrosomal signal intensities in NBs using fixed larval brains (Fig. 5e,f, Supplementary Figs 9b–d and 12e), confocal images of the ...
In case a compound of the present invention is depicted in form of a chemical name and as a formula in case of any discrepancy the formula shall prevail. An asterisk is may be used in sub-formulas to indicate the bond which is connected to the core molecule as defined, for example a ...
In order to change the pH value, 0.1 M ammonium hydroxide (NH4OH) was added drop wise to every sample. Values of pH were monitored by using a UB-10 pH/mV meter (Denver Instrument, New York, NY, USA). For the temperature tests the samples were exposed to thermal treatment at 60 °C...
To solve the question, we need to determine which of the two compounds, AgCl or PbCl2, forms a white precipitate that is soluble in excess ammonium hydroxide (NH4OH).1. Identify the Compounds: The two compounds given are AgCl (
pastoris parent strain, integration into the correct locus was verified by colony PCR. All overexpression (OE) cassettes, the average increase of expression achieved by overexpression (OE strength), the used parent strains and the abbreviated names given to the respective strains can be found in ...