CD4-/- or immunocompetent C57BL/6 mice were infected with the clone-13 strain of LCMV and shedding of virus from body fluids sampled. Although we detected high titers of virus in the saliva and blood of all infected animals, we observed higher levels of transmission to susceptible mice from...
Mice acutely infected with the Armstrong strain of lymphocytic choriomeningitis virus (LCMV) mount a robust immune response that rapidly clears the infection. In contrast, the use of the Clone 13 strain of LCMV results in a chronic infection, eliciting the progressive exhaustion of effector CD8+ T...
As expected, the ‘distal’ promoter (exon 1a) presented the primary origin of Il1rl1 transcripts in Th2 cells (type 2 promoter). To assess the usage of the type 1 promoter in vivo, we next transferred naive lymphocytic choriomeningitis virus (LCMV)-specific T-cell receptor (TCR)-...
we performed single-cell (sc) RNA sequencing of CD44+PD-1hiCD8+T cells isolated from chronically infected wild-type (WT) mice after infection with the clone 13 strain of lymphocytic choriomeningitis virus (LCMVCL13) (Supplementary Fig.1a). To avoid a bias due to active cell...
The recipient mice are subsequently infected with a strain of LCMV causing either acute (strain Armstrong) or chronic infection (strain clone 13), which induces P14 activation and acute or chronic infection-specific differentiation programs. Prior to infection, naive (unstimulated) P14 T cells ...
, 2002). Indeed, myeloid DCs (CD11chighLy6C−B220−) isolated from spleens of mice infected with LCMV Clone 13 (Cl 13) released high levels of type I IFN (Diebold et al., 2003). Similarly, influenza virus lacking NS1 as well as synthetic dsRNA poly(I:C) induced substantial amount...
can enhance the control of chronic infection with LCMV clone 13, mediated by CD4 + T cells [231]. IFN-Is have also been observed to dampen T cell responses by promoting the expression of immunosuppressive genes such as IL-10 and programmed cell death 1 ligand 1 (PDL1) to facilitate...
However, the role of IL-33 in the ROCV-induced host CNS-damage was not addressed yet. In human, rhinovirus infection induced asthma exacerbation dependent of IL-33 and the production of type 2 cytokines IL-4, IL-5, and IL-13. The authors state that IL-33 induction directly correlates ...
L. Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. J. Immunol. 136, 2348–2357 (1986). CAS PubMed Google Scholar Mosmann, T. R. & Coffman, R. L. TH1 and TH2 cells: different patterns of lymphokine ...
HeLa—Mutant HeLa clone that is hypoxanthine D98/AH.2 guanine phosphoribosyl transferase-deficient (HGPRT−) (also HeLa s) kindly provided by Eric J. Stanbridge [Department of Microbiology, College of Medicine, University of California, Irvine, Calif. (USA)] and reported in Stanbridge et al....