The primary mechanisms by which TRs play a critical role in the structural stability of genetic materials during the cell cycle are described subsequently: Replication fork stabilization TRs, consisting of repeated DNA sequences adjacent to each other, can stabilize the replication forks during DNA ...
Models for the cell division cycle in bacteria have been based largely on either species displaying symmetric binary fission or on Caulobacte r displaying asymmetric division correlated with a prosthecate stalk [7–10]. Other asymmetric models have also been described in preliminary terms [11]. In...
The network that regulates the mitotic entry of the cell-cycle in eukaryotes also makes a decision before it induces early mitotic processes. Here we show that the switch from inactive to active forms of the mitosis promoting Cyclin Dependent Kinases is driven by a system that is related to ...
We note that these cell cycle times are longer than all previously described cell cycle durations in Arabidopsis (reviewed in [17]). Since this data was acquired under dark conditions, we tested if the cell cycle duration is shorter under light conditions. We found that the cell cycle was in...
Cell survival and death were assessed in β-galactosidase-expressing neurons based on the integrity of neurites and integrity of the nucleus as determined by the DNA dye bisbenzimide (Hoechst 33258). Indirect immunofluorescence was carried out as described (Bonni et al., 1999). Cell counts were...
Cell cycle regulation in the postmitotic neuron: oxymoron or new biology? Adult neurons are typically described as permanently postmitotic but there is probably nothing permanent about the neuronal . Rather, it appears that these... K Herrup,Y Yang - 《Nature Reviews Neuroscience》 被引量: 595...
The cells were subsequently lysed to make the cell extracts that were used to perform RIP as described in Methods [29] and outlined in Figure 2. We used RT-PCR to analyze the RIP, I and S fractions for P27. cDNA was made using equal amounts (25 ng) of RNA from each fraction (...
Cell counts were determined using a micro-cell counter CC-108 (Sysmex, Kobe, Japan). Cells in a logarithmic phase of growth were used for the study and are described as follows. Establishment of the Transplantation Tumor Model and Grouping HT-29 cells were transplanted via subcutaneous ...
the respective mRNA/proteins are regulated by the host cell cycle, we choseG.thetaas a material and selected mRNAs (quantified by quantitative RT-PCR) and proteins (quantified by immunoblotting) that are related to the cell cycle, nucleomorph replication and chloroplast division, as described ...
Cell growth, a process that coordinates with cell cycle during cell doubling, is defined as an increase in cell mass and size5. This leads to lower surface area to volume ratio in cells and spurs cells to divide. A key regulator of cell growth is Akt (also known as protein kinase B or...