For example, cigarette smoke extract can induce vascular smooth muscle cell death via ferroptosis14. IA onset is associated with VSMC death, which leads to thinning of the media and an increased rupture risk15. SLC2A12 is expressed in VSMCs at both the mRNA and protein levels 16. Based ...
It is noteworthy mentioning that, in mammalian cells, DNA repair often favors NHEJ over HDR, with the HDR machinery largely restricted to the late G2 and S phases of the cell cycle (Kass and Jasin, 2010). As corollary, HDR-mediated genome editing is mostly restricted to cycling cells. ...
Extended Data Fig. 2 HCAR1 limits the CD8T cell mediated anti-tumor immunity in colorectal cancer, related to Fig.. a, Representative DNA sequencing data ofHcar1gene in intestinal epithelial cells, heart and liver tissues ofVil1-cre Hcar1fl/flmice at week 8.b, Representative immunoblotting...
Guo HS, Xie Q, Fei JF, Chua NH: MicroRNA directs mRNA cleavage of the transcription factor NAC1 to downregulate auxin signals for Arabidopsis lateral root development. Plant Cell. 2005, 17: 1376-1386. 10.1105/tpc.105.030841. Article PubMed Central CAS PubMed Google Scholar Gutierrez L, ...
Methods Mol Cell Biol. 1992, 3: 15-22. CAS Google Scholar Ioannou YA, Chen FW: Quantitation of DNA fragmentation in apoptosis. Nucleic Acids Res. 1996, 24: 992-3. 10.1093/nar/24.5.992. Article PubMed Central CAS PubMed Google Scholar van de Peer Y, De Wachter R: TREECON for ...
Interestingly, toxicants that exert their disruptive effects at the cell-cell interface, most notably in cell junctions (or cell adhesion sites) in testicular cells, such as Sertoli cells, germ cells, and Leydig cells, which in turn lead to defects in spermatogenesis and male reproductive ...
HiFi and Illumina sequencing were performed on one specimen from the Bukhangang River. We employed Pacific BioSciences (PacBio, Menlo Park, CA, USA) HiFi single-molecule real-time (SMRT) sequencing following the manufacturer’s protocol (Macrogen Inc.). The HiFi SMRT cell library was prepared ...
cell line, which was used as a reference of comparison. Bottom panel: Pie charts annotating the percentages of changes in HEYA8 eTADs with respect to PEO1 eTADs (left) and changes in PEO1 mTADs with respect to HEYA8 mTADs (right).cDistribution profile plots and heatmaps of the ...
This cell cycle arrest is executed by either of the two important cell cycle inhibitors, p21 or p16, and has so far not been reversible by any known combination of growth factors [4]. Triggers of cellular senescence By now, several other triggers to a replicative senescence-like irreversible...
Phenotypic data derived from high content screening is currently annotated using free-text, thus preventing the integration of independent datasets, including those generated in different biological domains, such as cell lines, mouse and human tissues. W