Q5™ High-Fidelity DNA Polymerase, T4 DNA ligase, restriction endonucleases, and DNA purification kits were purchased from New England Biolabs (NEB, UK). Plasmid isolation kits were purchased from Tiangen Biotech Co. Ltd. (Beijing, China). Yeast genome extraction kits were purchased from ...
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Regents were purchased from Sigma-Aldrich, New England Biolabs, Invitrogen, and Thermo Fisher Scientific. DNA primers were purchased from Sangon Biotech, Tsingke Biological Technology, and RuiBiotech. The synthesized peptides (from Sangon Biotech) were shown in Supplementary Table 14, and their ...
Adapters provided in the respective library kits were ligated to DNA samples with Quick T4 DNA Ligase (New England Biolabs) and samples were incubated at room temperature for 10 min. Purification and loading of adapted libraries on an appropriate flow cell (R9.4.1, ONT) was completed as ...
Crop yield is adversely being influenced very frequently due to biotic and abiotic stresses, globally. The insufficient yield and low nutritional value of the crops are due to the numerous ambient stresses. Which has challenged the nutritional security o
The BK Dunlop genome (a kind gift from Professor Michael Imperiale, University of Michigan) was excised from the pGEM7 Dunlop plasmid by BamHI (New England Biolabs) digestion and then recircularized using T4 Ligase (New England Biolabs). Vero cells were seeded into T75 flasks and transfected...
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The residues R331, L358, E379, D381, E384, W362, and H398 of BpGH16MLG were mutated to Ala by the Q5Site-Directed Mutagenesis Kit (New England Biolabs, UK, catalogue no. E0554S). Wild-type plasmid BpGH16-pET28a(+) was used as a template, and mutagenic forward and reverse prim...
biolabs | Beacon Analytical Systems | biotechrabbit | broadpharm | prime synthesis | BioResource Technology | Braintree scientific | alexotech | anchor molecular | analytical | AD Instruments | ab biosciences | apohtech | Abeomics | alstem | am systems | avantor | ASAHI | AQIX | Aquatic ...
biolabs | Beacon Analytical Systems | biotechrabbit | broadpharm | prime synthesis | BioResource Technology | Braintree scientific | alexotech | anchor molecular | analytical | AD Instruments | ab biosciences | apohtech | Abeomics | alstem | am systems | avantor | ASAHI | AQIX | Aquatic ...