a, The expression level (transcripts per million, TPM) of genebenacross different tissues forHylyphantes(n = 4 samples for abdomen, 3 for brain, 11 for legs, 3 for silk glands and 3 for venom glands).b, Genebenshowing up-regulated expression in web-building spider (n = 3 sa...
MacCalman CD, Brodt P, Doublet JD, Jednak R, Elhilali MM, Bazinet M, Blaschuk OW (1994a) The loss of E-cadherin mRNA transcripts in rat prostatic tumors is accompanied by increased expression of mRNA transcripts encoding fibronectin and its receptor. Clin Exp Metastasis 12: 101–107. Ar...
Independent of the strategy, RNA-Seq can accurately estimate gene expression (at the transcripts level) of all genes, in parallel, under different conditions and allows the discovery of new genes and transcription patterns, helping to understand cell function and metabolic mechanisms. In addition to...
The rest represented ncRNAs (noncoding RNAs), pseudogenes, snoRNAs (small nucleolar RNAs), snRNAs (small nuclear RNAs), and others (i.e., novel genes/transcripts, putative genes, intronic sense/antisense transcripts, antisense transcripts, etc.) (Figure 3a and Supplementary Table S1). Through...
Studies have revealed high abundance of some circular transcripts, cell- and tissue-type specific expression, evolutionary conservation, and exceptional stability [132,133,134]. The majority of detected circRNAs are exonic circRNAs derived from protein-coding genes and the most frequent mechanism of ...
Quantitation of MRNA Transcripts and Proteins Using the BD RhapsodyTM Single-Cell Analysis System. In Single Molecule and Single Cell Sequencing; Advances in Experimental Medicine and, Biology; Suzuki, Y., Ed.; Springer: Singapore, 2019; pp. 63–79. ISBN 9789811360374. [Google Scholar] Goldstein...
In the first step, one author (MS) reviewed the transcripts line by line. Interview sequences or single words were assigned to broad categories. Once the interviews had been transcribed verbatim, the research group (JD, FH, CD, and MS; SL), including health psychologists, a master of scien...
Pseudobulk analysis also revealed differentially expressed genes that were not identified by bulk RNA-seq approach. Some of these upregulated transcripts identified using pseudobulk spatial transcriptomics include genes related to chromatin binding (Ncoa2,Polg,Smc3, Bcl6,Jdp2, Sp3), protein kinase inh...
(ncRNAs) and mitochondrial protein coding genes, the resulting dataset consists of 2604 individual spots, with an average of ~4125 genes and ~11801 unique transcripts per spot (Supplementary Fig.1b). First, we deconvolved the spatial transcriptomic dataset using non-negative matrix factorization (...
The introns were defined using the “collapse” option in which transcripts of the same gene are first collapsed by taking the union of all the exonic regions within a gene and labeling the remaining non-exonic parts as introns. In contrast to the alternative “separate” strategy in which ...