The Agilent 2100 bioanalyzer system as compliant tool in Life Sciences – Part 1 Technical OverviewAgilent, The
内容提示: Glycoprotein sizing on the Agilent 2100 bioanalyzerApplicationAbstractGlycoproteins analyzed either by traditional SDS-PAGE or on the Agilent 2100 bioanalzyer frequently migrate at a different size than anticipated from the known molecular weight. Because the glycanattachment can keep the protein...
Analysis of PEGylated Proteins Using the Agilent 2100 Bioanalyzer System Application Note Biopharmaceuticals Authors Suresh Babu CV, Ravindra Gudihal, Agilent Technologies India Pvt. Ltd, Bangalore, India. Sundaram M Palaniswamy, Umamaheshwari S, Suneel Basingi, GangaGen Biotechnologies Pvt. Ltd, ...
In this application note the performance of the Agilent 2100 bioanalyzer is compared to the most commonly used techniques for RNA separation,detection and quantification. Dilutions of ribosomal RNA from E. coli and poly (A) RNA from mouse kidney were used to determine performanceover a broad conc...
The Agilent 2100 Bioanalyzer™ (Agilent Technologies, Santa Clara, CA, USA) and a Qubit dsDNA HS Assay Kit (Life Technologies, Carlsbad, CA, USA) were utilized for the library purification and quantification following the manufacturer’s instructions [6]. Next, paired-end sequencing was ...
Beverly, MA USA Low input DNA size selection on the Pippin Prep System using the Agilent 2100 Bioanalyzer with the Agilent High Sensitivity DNA kit Application Note Genomics [FU] 100 80 60 40 20 0 -20 [FU] 30 25 20 15 10 5 0 -5 -10 100 Input load (ng) 250 150 50 15 150 200...
This product is no longer available on Biocompare. Product Specs Item2100 Bioanalyzer Systems CompanyAgilent Technologies Catalog NumberG2939BA
(Invitrogen, USA) and 1% agarose gel electrophoresis. The detail of DNA library construction was described in Supplementary File1. The final DNA library was determined the average insert size using the Agilent 2100 Bioanalyzer (Agilent Technologies, USA) and quantified by ABI StepOnePlus Real-Time ...
(Thermo Scientific, USA). Sequencing libraries were validated using an Agilent 2100 Bioanalyzer (Agilent Technologies, United States), and quantified using a Qubit Fluorometer (Qubit 2.0, Thermo Scientific, USA). Finally, paired-end sequencing was conducted using an Illumina HiSeq 2500 platform (...
The total RNA purity and quality was detected using the Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA, USA) and RNA Nano 6000 Assay Kit, respectively. For circRNA sequencing, firstly, we take approximately 5 µg high quality RNA per sample as input material and use the 15 U Rnase ...