We removed such outliers from both amino acid and nucleotide alignments. We used analysis of variance to assess whether there were significant differences in the number of orthologs in our primary dataset based on taxonomic characteristics of taxa or the data type of those sequences (AHE, ...
Here we report that pyrrolysyl-tRNA synthetase (PylRS) and certain PylRS variants accept α-hydroxy, α-thio and N-formyl-l-α-amino acids, as well as α-carboxy acid monomers that are precursors to polyketide natural products. These monomers are accommodated and accepted by the translation ...
This allele represents an increased risk seen across different ethnic groups of 3-fold for heterozygous carriers and up to 15-fold for individuals who are ε4 homozygotes, when compared with ε3 homozygotes (Ashford, 2004). APOE is known to act in a dose dependent manner in AD: the ...
Let M be a multiple sequence alignment, where its first row represents the protein under study. Every residue of that protein is then uniquely determined by its column. In what follows, we identify the residues of the protein with their columns of the MSA. Grosse et al. [35] pointed out...
These findings indicate that ompA and LPS mutations do not alter the conjugation efficiency of the closely related IncFII-type plasmid R100, in which the amino acid sequence of the TraN central region is highly divergent from that of F-encoded TraN [59,77,80]. Mating pair stabilization ...
Chromatographic separation of the metabolites was performed using an ACQUITY BEH C18 column (100 mm × 2.1 mm i.d., 1.8 µm; Waters, Milford, USA). The mobile phases consisted of 0.1% formic acid in water:acetonitrile (95:5, v/v) (solvent A) and 0.1% formic acid in acetonitril...
Such microbiome-metabolite associations reported in individual studies of the human gut, however, raise several questions. First, it remains unclear what do such associations mean biologically, and whether or not they reflect underlying mechanisms. In fact, our lab has recently shown that microbe-meta...
Which procedure represents the safest technique to be used in diluting concentrated acid?
The human genome holds an extraordinary trove of information about human development, physiology, medicine and evolution. Here we report the results of an international collaboration to produce and make freely available a draft sequence of the human geno
However, the Gibbs free energy change Δ𝐺W1𝑗 can be affected by pH and ionic strength, which could be modified by the presence of cosolvent molecules, if they possess acid-base or ionic properties (such as for some of the modified sugars exploited in this work; see Table S1). In ...