aThe variable region where the information is encoded will be cloned between conserved sequences that contain primer-binding domains. To read the DNA-encoded information, one only needs to perform PCR and sequence the fragment. 易变的区域,信息被输入将被克隆在包含底漆束缚的领域的被保存的序列之间。
The results of our studies suggest that only the 5'-proximal PBS has been used to initiate . On the basis of our results, a mechanism is proposed for the generation of a dual PBS, which provides new insights into . 展开 关键词: HCV, vero cell, cell clone, virus binding, susceptibility...
Previous results using a SELEX (Systematic Evolution of Ligands by Exponential Enrichment)-based approach that selected DNA primer-template duplexes binding with high affinity to HIV reverse transcriptase (RT) showed that primers mimicking the 3′ end, and in particular the six nt terminal G tract...
As a proof of principle, we have designed a universal primer, rbs_B0034, that contains a ribosomal binding site (RBS; BBa_B0034) and that can be used in PCR to amplify any coding BioBrick that starts with ATG. We performed test PCRs with rbs_B0034 on 31 different targets and found...