02、分选的Naive CD4+ T cells 加入包被孔内 a) 96 孔板加入 1×105 细胞(200μl培养体系)b) 48 孔板加入 2×105 细胞(500μl培养体系) 03、配置分化培养基 4μg/ml anti-Human CD28 + 10ng/ml Human IL-2 根据需要分化的方向额外添加以下成分:1) Th1细胞: IL-12 (50 ng/mL) + anti-human ...
Sakaguchi S., et al., (1995) Immunologic self-tolerance maintained by activated T cells expressing IL-2 receptor alpha-chains(CD25).Breakdown of a single mechanism of self- tolerance causes various autoimmune diseases[J]. Journal of Immunology, 155( 3) : 1151-1164.6. Betts G., et al., ...
Researchers futher hypothesized that among IECs, it is Lgr5+ ISCs that interact with Th cells via MHCII, they sorted EpCAM+ cells, which contain Lgr5+ ISCs and cultured them with DQovalbumin. Compared to EpCAM+ GFP cells, co-culture with Lgr5-GFP+ ISCs or DCs caused substantially higher...
少数Th细胞被称为Th3细胞,它除分泌大量的IL-4和IL-10外,主要高表达TGF-β,但是不分泌IL-2和IFN-γ,可以下调抗原递呈细胞(antigenpresenting cells,APC)及Th1细胞的活性,起免疫抑制作用。 Th1, Th2和Th3细胞均由前体细胞Th0分化而来。未接触抗原的T细胞被称为Th细胞前体(Thcell precursor),他们通过接触天然免疫...
细胞毒性T细胞(Cytotoxic T Cells,CTL)是机体获得性免疫的主要细胞亚群,细胞表面表达CD8抗原,被称为CD8+T细胞。CTL可以识别、结合并杀伤被细菌或病毒感染的细胞及肿瘤细胞。CTL细胞在骨髓与胸腺中发育成熟。 DC(Dendritic Cells,树突状细胞) 树突状细胞(Dendritic Cells,DC)是...
辅助性T细胞（helper T cell)，简称Th细胞，能分泌多种细胞因子。Th细胞主要分为Th1细胞与Th2细胞两种，其中，Th1细胞参与细胞免疫和迟发性超敏性炎症反应;Th2可辅助B细胞分化为抗体分泌细胞，参与体液免疫应答。艾滋病病毒能特异性地破坏Th细胞，导致患者免疫系统瘫痪。Th细胞在免疫系统中起重要作用，有...
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[11] Lu, Y. et al. Th9 cells represent a unique subset of CD4(+) T cells endowed with the ability to eradicate advanced tumors. Cancer Cell 33, 1048–1060 e1047 (2018). [12] Shen, Y., Song, Z., Lu, X. et al. Fas signaling-mediated T...
1、Wang, Hongxing et al. T-Cell Immune Imbalance in Rheumatoid Arthritis Is Associated with Alterations in NK Cells and NK-Like T Cells Expressing CD38. Journal of innate immunity vol. 14,2 (2022): 148-166. 2、Ding Y, Wang Y, Zhang Y, et al. Alpha-linolenic acid improves nasal mucos...