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Guidance on how to modify the amino acid sequences of the protein domains disclosed herein can also be obtained by producing a protein alignment of a reference protein (e.g., SEQ ID NO:2, SEQ ID NO:4, or SEQ ID NO:6) with other related polypeptides. For instance, the reference protei...
coli codon optimized h-KYNU gene (nucleotides 97-1488), a stop codon (nucleotides 1489-1491), and a C-terminal BamHI restriction enzyme site (nucleotides 1492-1497) (see, SEQ ID NO: 2). The aforementioned restriction enzyme sites were used to clone the assembled gene into a pET-28a+ ve...