We offer several plasmid and viral delivery options for the Tet-On 3G system, plus promoters that allow for consistent long-term expression of the Tet transactivator.Tet-On 3G systems Tet-On 3G—lentiviral Tet-On 3G—retroviral Adeno-X Tet-On 3G inducible expression system Tet-On 3G ...
The TetR plasmid was stably transfected into the RepTor™ cell lines, to save you time and effort. Upon transfection of the RepTor™ cells with a pTiGer-GOI plasmid, TetR binds to TRE and blocks the transcription of the GOI [2]. Addition of doxycycline, a synthetic analog of tetracyclin...
construct the Tet-On inducible expression plasmid of methyl-binding protein 1 (MBD1) and 293T cells, to explore the effect of doxycycline (Dox) on the expression of MBD1, and to reflect the regulatory mechanism of MBD1 by analyzing the CpG methylation...
HH-shRNA-082 Tet-pLKO-neo诱导型慢病毒shRNA表达载体G418抗性 HH-shRNA-077 Tet-pLKO-puro-Scrambled (Plasmid #47541)慢病毒的诱导型shRNA对照质粒NC HH-shRNA-076 TET-pLKO.1 PURO shLuc (Plasmid #83092)慢病毒的诱导型shRNA对照质粒NC HH-shRNA-075 tet-pLKO.puro_shGFP (Plasmid #110421)慢病毒的...
3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection 质粒类型: 慢病毒载体 载体大小: 10633 bp 5'测序引物: GGCAGGGATATTCACCATTATCGTTTCAGA 载体抗性: Ampicillin 别名: Tet-pLKO-puro 筛选标记: Puromycin (Empty Backbone)3rd generation lentiviral plasmid for inducible expr...
The Advanced TRE Response Plasmid: pTRE-TightpTRE-Tightcontains a modified TRE that both reduces basal expression and promotes tight binding to the Tet-Advanced transactivator proteins during induction to permit specific and high-level transcription of your downstream gene. ...
Tet—onregulatingplasmidwastransferredintoA519cellsundertheDoxinducedTetonregulatingsystemand stableexpressionofTet—onwasestablishedinA549cellsthroughG418selection.TheresponseplasmidsofrecombinantPTKHyg andPTRE—EGFP—esplweretransferredintopositiveA5d9/Tet—oncells,andstableexpressionofesplwasestablishedthroughhy— ...
To avoid the phenomenon of transgene down-regulation, we have employed strong, constitutive promoters and compared this expression system with the inducible expression system incorporated in a single plasmid vector or in lentiviral vectors. Plasmid-based transgene expression directed by the constitutive, ...
Note that plasmid, retroviral, and adenoviral Tet-On 3G inducible expression systems are also available. Lenti-X Tet-On 3G inducible expression systems with CMV regulator vectors Core elements common to every vector ProductRegulator vectorResponse vector Cat. # 631187 Lenti-X Tet-On 3G Indu...
Tet-On 3G Вector Set (with ZsGreen1) 商品说明书 Certificate of Analysis Takara Bio USA, Inc.1290 Terra Bella Avenue, Mountain View, CA 94043, USA U.S. Technical Support: *** United States/Canada 800.662.2566 Asia Pacific +1.650.919.7300 Europe +33.(0)1.3904.6880 Japan +81.(0)...