Tax, under the TET-promoter (TET-TAX) was provided by Warner Greene. The reverse tet activator, coupled to the granzyme B promoter (GZB-rtTA) , drives Tax in activated T and NK cells in a doxycycline (dox)-inducible manner. Two additional transgenes were included for non-invasive imaging...
pTiGer-mcs plasmids: Cloning of a gene of interest (GOI) downstream of a Tet-inducible promoter pTiGer-reporter plasmids: Tet-inducible expression of a reporter gene (controls) Features The TiGer system has been specifically designed to allow the controlled expression of cytotoxic genes. Minim...
Tet-One Systems "All-in-One" Design Before the Tet-One Systems were developed, Clontech's Tet-On and Tet-Off® products all required two separate vectors to introduce the transactivator protein and the inducible promoter controlling your gene of interest, respectively, into your target cells. ...
The Tet gene expression system functions when a recombinant tetracycline-controlled transcription factor (either tTA for Tet-Off or rtTA for Tet-On) binds to theTet-op promoter, subsequently driving the expression or controlling the inhibition of the target gene (see figure below). Gene expression ...
Description The Tet-On 3G Vector Set (with ZsGreen1) is used to create tightly regulated and highly responsive tetracycline (Tet)-inducible mammalian expression systems that are turned on by the addition of doxycycline to the culture medium. The Tet-On 3G Vector Set (with ZsGreen1) allows the...
Inducible tet-on miR-204 lentivirus (2x25ul) Price CN¥8,330.00 It has been observed that microRNA miR-204 plays a role in the regulation of cancer metastasis. This lentivirus expresses miR-204 under the control of the Tet promoter. Virus:Inducible tet-on miR-204 lentivirus Titer (approx...
enhancedstabilityTetOn®/Off®AdvancedInducibleTet-On®/Off®AdvancedInducibleExpressionSystemsBasedontwoelements:RegulatoryandResponsePromoter activation allows tunable regulation high fold‐inductionPromoter activation allows tunable regulation, high fold‐inductionGeneactivitycontrolledbysupplyorwithdrawalofDoxTet...
They are readily transfectable with a pTiGer plasmid, carrying a gene of interest (GOI) cloned downstream of the tet-inducible CMV-EF1-TRE promoter. GOI expression is induced upon addition of doxycycline, a synthetic analog of the tetracycline antibiotic, leading to derepression of the CMV-EF1-...
The adaption of the inducible, tetracycline-dependent Tet-on promoter system from Aspergillus niger achieved a controlled overproduction of this toxic metabolite and a functional characterization of each cluster gene in F. fujikuroi. Overexpression of one of the two cluster-specific transcription factor ...
United States Patent US10590434 Note: If you have problems viewing the PDF, please make sure you have the latest version ofAdobe Acrobat. Back to full text