Finally, slides were mounted with Permafluor (Thermo Scientific, UK) and fluorescent images captured using an Olympus BX61 microscope (Olympus). Antibody dilutions are listed in Supplementary Table 2. BrdU incorporation Before end of ex vivo culture period, tissue fragments were cultured with BrdU ...
Finally, the samples were examined using a Zeiss LSM780 confocal microscope (Zeiss, Jena, Germany). Functional analysis of the role of lnc117962 in male fertility The primer design, synthesis, quality detection, and RNAi of lncRNA were executed following the procedures described in the preceding ...
Spawning of the recipient fish with wild-type females produced viable offspring demonstrating donor-derived traits. All F1 offspring displayed a green fluorescent signal under the microscope, expression ofyfpwas confirmed with RT-PCR amplification, and most importantly, F1 individuals developed and matured...
The sections were stained with H&E staining, and the pathological changes of the testis tissue and the amount of Leydig cells and spermatogenic cells were observed under an orthotopic optical microscope. 2.4. Immunofluorescence Testicular tissues in 4 % paraformaldehyde fixative were fixed for 72 h,...
Id4 Marks Spermatogonial Stem Cells. To examine whether Id4 marks SSCs, we labeled Id4+ cells and their progeny by intraperitoneal injection of TM into a cohort of 10-day-old Id4-CreERT2-tdTomato; ROSA26-flox-stop-lacZ mice. The testes were isolated and stained with X-gal 3 ...
Under an Elsevier user license open archiveThe blood-testis barrier (BTB), formed between adjacent Sertoli cells, undergoes extensive remodeling to facilitate the transport of preleptotene spermatocytes across the barrier from the basal to apical compartments of the seminiferous tubules for further develop...
Finally cells unfixed or fixed in methanol for 30 min, were mounted on slides and observed under a coverslip with a Zeiss fluorescence microscope with epi-illumination. The number of labeled cells was recorded for each field and the percentage of fluorescent cells was calculated after counting 300...
Slides were washed with PBS as described previously and then viewed under an epifluorescence microscope (Axioskop 2plus; Zeiss, Germany). Conservation of the Protein Across the Species To study the conservation of the cognate protein across the species, indirect immunofluorescence (IIF) was performed...
{dTSSK};dTSSK−/−flies. dTSSK−/−flies exhibit male sterility, and replenishment of wild-type dTSSK protein restores male fertility.gLive imaging of seminal vesicles of the indicated genotypes stained with DAPI (white). Sperm nuclei (green) are labeled with protamine Mst35Bb-GFP. No...
detection levels were set identically for each section treated with the same primary and secondary antibody in an individual experiment to ensure consistency. Cells with a distinct signal evident at 6000 or higher on the intensity scale were labeled as ‘Hi’, cells with a signal between 3000 and...