For 6-O-desulfation, 5 mg of the pyridine salt of HS was placed into a glass test tube containing 5 ml of DMSO/2% pyridine (100 μl) and heated at 100° C. for 9 hours. The solution was then diluted 50:50 with deionized water and dialyzed against deionized water and lyophilized. ...
9]. The Coombs’ test was established in 1945 by Robin Coombs, a veterinarian and immunologist at Cambridge, United Kingdom, although Italian scientists reported on the conceptual basis for this test even earlier [10]. The DAT detects bound antibodies (and complement) on the surface ...