One of the requirements of our tool is the introduction of LoxPsym sites at the promoter and terminator regions, which can influence expression regulation of the targeted gene. Therefore, we tested the effect of inserting LoxPsym sequences at different positions in the GEM-blocks, usingyECitrinea...
One of the requirements of our tool is the introduction of LoxPsym sites at the promoter and terminator regions, which can influence expression regulation of the targeted gene. Therefore, we tested the effect of inserting LoxPsym sequences at different positions in the GEM-blocks, usingyECitrinea...
Two apparent rho-independent transcription terminator structures within the coding sequence of aceK have been destroyed to access their roles in the differential expression between aceA and aceK in the glyoxylate bypass operon of E. coli. The effect of mutations on the expression of aceK was evalu...