gNoexnt,ththeeNco-tnetrrmibiuntailonreogfioenacahndamthineothacreide to the free previously identified amino acids (R3.28, Q3.29, K7.36) as references21. The free energy profile identified Lys39 as key N-terminal amino acids contributing to ligand binding whilst validating the contributions of ...
(PoEf) NanFdκBt,henuacclteivaartfioanctoorf oNfFaκcBti,vnautecdleaTr cfealcltso(rNoFfAaTc)t,ivaanteddGTAcTeAll-s4 (iNnFrAesTp),onansed to GPAETwAa-s4 einvarleusaptoendsebytowPeEstewrnasbelovta.luPatEedtrbigygewreesdtetrhne bplohto.sPpEhotrryiglagteiroend otfheGAphToAs-p4h, oNr...
(y.cohFduTcrtineh)oNorbli)eeir,HcoecolvmrtifioetwwdrfatmreLFiwrmiooaevmieo)Caainmretceandmahwsss---a,lshteyyrieatpicy)lthbsa,huLyircdwytreCleoilrsftsfiarrit,ateysaseMymdrcd(rpetteLiuienudbvnrCneryteee)--- thylstyrene) w(PitChM2,S2)-awzaosbissyisnotbhuetsyizroenditbriylec(oAnIvBeNn...
The supernatant was discharged and the pellet was resuspended in 10 mL 50 mM Tris buffer (pH 8) prior to sonication. Sonication was carried out for 4 min with 30 s interval on ice by using Branson Sonifier 250 (output two, duty cycle: 30) (Branson, Danbury, CT, USA). The cells ...
fμoorH/bTo)tihn cFoigmupreou7,nwdsh.icThhwuse,rethfietteisdottoroapZiceesmimanulpatluiosnaxoifalthe mazgernoe-tfiicelsdusspceliptttiinbgiliHtyamofil1toannidan2(gĤen=eDra(Ŝtez2d−thSe(Sp+lo1t)s/3o)f+thμeBgeHneŜr,gwyhveerersDusistotthael sapxiianl sahnoiswontroinpyFiogf...