scRNA-seq data have been submitted into the Gene Expression Omnibus at GSE138941. Code availability Source code for next-generation sequencing data processing and scRNA-seq is available at https://github.com/imallona/stem_cells_hippocampus_jessberger_lab/. References Gage, F. H. Adult ...
One of the biggest challenges in tissue engineering and regenerative medicine is to ensure oxygen supply of cells in the (temporary) absence of vasculature. With the vision to exploit photosynthetic oxygen production by microalgae, co-cultivated in close
Around 5-10% of people with Leber's congenital amaurosis have mutations in this gene.The first study,1 carried out in the United States, treated 15 people with RPE65 associated Leber's congenital amaurosis. The results showed rapid improvement in light sensitivity that was detectable within the...
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CpG-reduced, CMV-based plasmid DNA constructs encoding human α-galactosidase A and factor IX were injected into C57Bl/6, BALB/c, and CD1 mice using hydrodynamics-based delivery of plasmid DNA (pDNA), and gene expression was monitored for 6 months. Linearized and supercoiled pDNAs were compar...
Genetic counseling was provided to each proband after obtaining informed consent according to a protocol approved by the IRB at INNN. We extracted DNA from peripheral blood samples and analyzed the coding-sequence, promoter region and all exon-intron boundaries of the VHL gene for mutations, as ...
There was statistical difference between them (Chi-Square = 71.168, p < 0.001).The Mann–Whitney adjusted by Bonferroni Pairwise comparisons were performed by U-test: the PSAI score of patients with 5α-RD2 deficiency, PAIS, and NR5A1 gene mutation (assigned as male) were higher ...
Extracted DNA was used to perform PCR to amplify CHD gene fragments with the primer pair P2 (5′-TCTGCATCGCTAAATCCTTT-3′)/P17 (5′-GAAGAAAATAATTCCAGAAGTCCA-3′) that has previously been developed for sexing zebra finches (Arnold et al., 2003). All PCR reactions were run in a final...
i MetaCore enrichment on gene sets characteristic to SBCs. P values are calculated by hypergeometric test. j Ridge plot showing the expression of the SBC signature in the indicated clusters of mouse mammary epithelial scRNA-seq data28. k UMAP plot depicting the SBC subclusters in SD rats ...
PCR was performed to investigate the protection of DNA by amplification of chromosomally encoded housekeeping gene G6PD (FigureS9). Primer sequences are listed in Supplementary Table1. Each PCR reaction was performed with a Bio-Rad C1000 Touch™ PCR system, using the following protocol: 25 μ...