One of the biggest challenges in tissue engineering and regenerative medicine is to ensure oxygen supply of cells in the (temporary) absence of vasculature. With the vision to exploit photosynthetic oxygen production by microalgae, co-cultivated in close
i MetaCore enrichment on gene sets characteristic to SBCs. P values are calculated by hypergeometric test. j Ridge plot showing the expression of the SBC signature in the indicated clusters of mouse mammary epithelial scRNA-seq data28. k UMAP plot depicting the SBC subclusters in SD rats ...
CpG-reduced, CMV-based plasmid DNA constructs encoding human α-galactosidase A and factor IX were injected into C57Bl/6, BALB/c, and CD1 mice using hydrodynamics-based delivery of plasmid DNA (pDNA), and gene expression was monitored for 6 months. Linearized and supercoiled pDNAs were compar...
homologous major capsid gene (LMPG_02241.2) in the 2000 isolates. Mechanistically, the observed recombination events in thecomKprophage most likely occurred through infection with one or more phages in one or more cells of the 1988 genotype, followed by subsequent recombination or phage replacement....
Xj-iis the number of differentially expressed genes that are not related to the termi(thusj-exclusive) andNj-iis the total number of genes that are not related to the termi(thusj-exclusive). Note that a given gene must be counted in one and only one of these cases and that summing ...
Genetic counseling was provided to each proband after obtaining informed consent according to a protocol approved by the IRB at INNN. We extracted DNA from peripheral blood samples and analyzed the coding-sequence, promoter region and all exon-intron boundaries of the VHL gene for mutations, as ...
The V4 region of 16 S rRNA gene was amplified and sequenced using Illumina MiSeq for all DNA extracts. The results obtained from the human DNA extracts were compared with each other and with the microbial DNA from the FACTS samples. Overall, we found that the presence of specific microbial ...
and bind to GRα resulting in a nuclear entry (Fig.2) [22] where GRβ modulates either positively or negatively the expression of target genes. GRβ has a very low level of expression compared to GRα [20] and acts mainly as a negative inhibitor of GRα-mediated gene modulation [23]...
The expression level of each gene was quantified in units of transcript per million (TPM), and fragments per kilobase of transcript per million fragments were mapped (FPKM) using RSEM (V1.2.15) with the default parameters. To compare the expression levels of different genes across samples, an...
scRNA-seq data have been submitted into the Gene Expression Omnibus at GSE138941. Code availability Source code for next-generation sequencing data processing and scRNA-seq is available at https://github.com/imallona/stem_cells_hippocampus_jessberger_lab/. References Gage, F. H. Adult ...