A new chelating cyclophane and its complexation with Ni2+, Cu2+, and Zn2+: Spectroscopic... Investigates the formation of a chelating cyclophane. Complexation of the cyclophane with nickel, copper, and zinc; Spectroscopic properties and allosteris... Inoue,Michiko,B.,... - 《Inorganic Chemist...
The supernatant was loaded onto a Ni-IDA column (Generon, Maidenhead UK) pre-equilibrated with 50 mM KPi (pH 8.0) loading buffer containing 250 mM NaCl and 10% glycerol, using a peristaltic pump (GE Healthcare, Little Chalfont UK). The column was washed with ~80 ml of loading ...
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