Enzyme activity values (expressed as nkatal/mg protein) are the mean of three biological replicates ± SD. p-value of two-way ANNOVA followed by a Tukey–Kramer test (**) p < 0.01. Exact p-values are provided in the Source Data file. d In contrast to PRX69, PRX62 is ...
These methods were validated using experimental data obtained by immobilizing the enzyme carbonic anhydrase II (CAII) and injecting mixtures of four CAII binders. Two main limitations remained in this framework. First, the number of available mixtures might be limiting when N is large. Second, ...
RNAseq analysis of co vs mono 39 for SA showed upregulation in several glycolysis-related genes (Additional file2: Table S1, Additional file4: Table S3, Table S5) and staphyloferrin biosynthesis genes. Interestingly, TCA-enzyme-coding genes and three succinate dehydrogenase subunits coding genes ...
serious damage was caused to the potato plant and the cell membrane had been damaged, resulting in a rapid increase in MDA content and inhibition of various enzyme activities, leading to a decrease in soluble sugar content
infantum. Finally, this analysis also revealed the up-regulation of an esterase-like protein, carbamoyl-phosphate synthetase (CPS), a short chain dehydrogenase and ubiquitin-conjugating enzyme-like proteins in TPS-induced AL, and the latter also under TS. Conclusions Absence of gp46 expression ...
2015). Amylopectin, the more abundant polymer of starch is synthesized by the coordinated actions of AGPase, soluble starch synthase (SS), starch branching enzyme (BE) and starch debranching enzyme (DBE) (Kang et al. 2013). Other genes coding for expansins have also been implicated in wheat...
cAMP concentrations were measured by using cAMP enzymeimmunoassay system (Cayman Chemical Company) as described previously [32]. HEK293T cells on 10 cm2 plates were transfected with 3 μg α2C-AR and six hours later were split into 12-well plates. The cells were serum starved for 24 h an...
(Q9R4G8, EC: 1.21.4.2) were identified. The first enzyme catalyzes the degradation of glycine to carbon dioxide, ammonia and methylene-THF, which could be further converted to acetate via the Wood-Ljungdahl pathway [44]. The second enzyme enables anaerobic degradation of glycine via the so-...
was simulated at 283, 298, 303, 308, and 313 K, again with 300 randomized replicas per temperature. The total simulation time of all enzyme EVB/MD calculations amounted to 3.2 μs. Fitting of enzyme activity curves to kinetic models was done with Gnuplot (http://www.gnuplot.info)....
A cell proliferation assay was performed using a WST-8 cell-counting kit (Dojindo,) and an albumin synthesis assay was performed by using an enzyme-linked immunosorbent assay (Rat Albumin ELISA Quantitation Set; Bethyl Laboratories,). Cell proliferative activity under culture temperature change Huh7...