the middle one third of each flag leaf was cut and immediately chopped into pieces and transferred to 6 mL of isopropanol with 0.01% BHT at 75 °C in a 50-mL glass tube with a Teflon-lined screw cap (Thermo Fisher Scientific, Inc., Waltham, MA...
https://ror.org/05abbep66grid.253264.40000 0004 1936 9473Department of MathematicsBrandeis University 415 South Street 02453 Waltham MA USAChristian Baadsgaard Jepsenhttps://ror.org/041hz9568grid.249961.10000 0004 0610 5612School of PhysicsKorea Institute for Advanced Study 85 Hoegiro 02455 Seoul ...
Additionally, both SEM and the transmission electron microscope (Thermo Scientific Talos F200S G2S/TEM, Waltham, MA, USA) were employed to assess the morphology and size of the Mxene. Initially, MXene was dispersed in a minimal volume of absolute ethanol via sonication, followed by deposition...
The concentration of total RNA was obtained using the Qubit RNA BR Assay Kit (Thermo Fisher Scientific, Waltham, MA). The quality of the RNA was determined by the Agilent RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA). Five micrograms of total RNA was depleted of ribosomal RNA ...
Two small sampling holes in the lid of the jar are used for sampling at selected time points during the experiment. The anaerobic jar is designed with an empty space next to the two sample chambers, where an anaerobic sachet (Oxoid™ AnaeroGen™, Thermo Fisher Scientific, Waltham, MA, ...
The activation process involved mixing the sperm suspension with the activation fluid in a 15 mL conical tube at a 1:10 ratio, followed by vigorous mixing using a vortex mixer (basic model vortex oscillator produced by Thermo Fisher Scientific, Waltham, MA, USA). A 5 µL sample was ...
MethodsA custom chamber is designed to hold two 96-well cell culture plates in which cells can be irradiated from below using two UVA sources (KXL, Avedro, Waltham, MA) under the plates. Gas composition is controlled by adjusting nitrogen and oxygen gas flow rates into the chamber. Oxygen ...
Timothy O'Leary 0 1 2 Eve Marder 0 1 2 0 Volen Center for Complex Systems, Brandeis University , Waltham, MA 02454 , USA 1 References 1. Tang LS , Goeritz ML, Caplan JS, Taylor AL, Fisek M , Marder E: Precise temperature compensation of phase in a rhythmic motor pattern. PLoS ...
Scientific, Waltham, MA, USA) according to the manu- facturer's instructions. Briefly, a single mosquito was put in a 1.5-ml Eppendorf tube and efficiently ground to powder by first being frozen in liquid nitrogen and then crushed with a pestle. For lysis, 100 μl of DNAzol was added ...
The quantity and quality of total RNA were assessed using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and agarose gel electrophoresis. Due to low-quality RNA obtained from the testis of two control fish, the cDNA was generated for n = 6 control and n...