TEM-1 beta-lactamase is the most widespread enzyme to confer beta-lactam antibiotic resistance to gram-negative bacteria. It is a monomeric protein that requires no metals or cofactors for activity. TEM-1 beta-lactamase efficiently hydrolyzes most penicillin and cephalosporin substrates, including nit...
上海康朗生物科技有限公司生产的Recombinant β-lactamase TEM-1,最新报价:646元/mg,Recombinantβ-lactamaseTEM-1▼▲AboutthisAntibodyNameRecombinantβ-lactamaseTEM-1(Re
P7082-100mg Recombinant β-lactamase TEM-1 100mg - 说明书 1份 保存条件: -20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。 注意事项: 由于...
G. Mutants of the TEM-1 beta-lactamase conferring resistance to ceftazidime. J. Antimicrob. Chemother. 24, 103-110 (1989).Payne DJ, Marriott MS and Amyes SG. Mutants of the TEM-1 β-lactamase conferring resistance to ceftazidime. J Antimicrob Chemother 1989; 24:103-110....
Beta Lactamase TEM 1 is an enzyme encoded by the bla gene in E. coli, with its expression being constitutive. The resistance to β-lactam antibiotics is directly proportional to the number of copies of the bla gene and the enzyme synthesized. ...
TEM-1. Also responsible for the ampicillin and penicillin resistance that is seen in H.influenzae and N.gonorrhoeae in increasing numbers. Based upon different combinations of changes, currently 140 TEM-type enzymes have been described. Recombinant beta-lactamase TEM-1 contains 264 amino acids ...
It was found that BLIP has significant differences in binding affinities toward TEM-1 and SHV-1 beta-lactamases. 展开 关键词: Binding Free Energy TEM-1 beta-lactamase SHV-1 beta-lactamase BLIP Molecular Dynamics 会议时间: 03 June 2010 被引量: 6 ...
Crystallization of the 1:1 molecular complex between the beta-lactamase TEM-1 and the beta-lactamase inhibitory protein BLIP has provided an opportunity to... NCJ Strynadka,M Eisenstein,E Katchalski-Katzir,... - 《Nat Struct Biol》 被引量: 418发表: 1996年 Emergence of clinical isolates of...
The TEM-1 beta-lactamase is a globular protein containing 12 proline residues. The folding mechanism of this enzyme was investigated by kinetic and equilibrium experiments with the help of fluorescence spectroscopy and circular dichroism. The equilibrium denaturation of the protein induced by guanidine ...
A restriction fragment of plasmid pBR322 bearing the TEM-1 beta-lactamase structural gene was electroeluted from agarose gels after digestion with EcoRI and HinfI. The 1-kilobase fragment was 32P-labeled and used to examine genetic relationships with nucleic acids encoding seven other beta-lactamas...