Samples were then washed in Saline Sodium Citrate with 0.1% Tween-20 (SSCT), before incubation in Blocking Reagent (Roche). Antibody labelling occurred overnight at 4 °C in Maleic Acid Buffer with Tween-20 (MABT), using anti-DIG-ALP (0.2 µl/ml) (Roche). This was followed by a ...