Application of gene knockdown techniques in the pinewood nematode Bursaphelenchus xylophilus.Park, Jung EunPark, Jung EunLee, Kyong YunLee, Kyong YunLee, SeJinLee, SeJinKoo, HyeonSookKoo, HyeonSook
solutions containing genetic material or knockdown constructs are delivered into the blastomeres: the embryonic cells sitting atop the yolk of the newly fertilized egg. Delivery into the cytoplasm is achieved
Interestingly, in the presence of a DUSP6 inhibitor, PICSAR knockdown had no effect on extracellular signal-regulated kinase 1/2 activation, indicating that DUSP6 may be the link between PICSAR and the extracellular signal-regulated kinase 1/2 signaling pathway (Piipponen et al., 2016). The ...
A major goal of the study of neurodevelopment is to figure out how cell identity and morphology are determined. To study the genetic control of neuron development, these researchers delivered gene knockdown constructs into the developing chick nervous system via electroporation. Electroporated neurons ...
[15,16]. Indeed, in human ESCs, neurogenesis and differentiation are blocked by siRNA-mediated knockdown of individual lncRNAs [17]. The nuclear lncRNAMALAT1, enriched in post-mitotic neurons, is known to regulate synaptic gene expression [18], and the CNS-enriched lncRNAPauparacts intrans...
The particular antisense oligonucleotide that we used is not yet in clinical trials, but there is potential scope for this. However, we first need to identify the full set of genes involved that could potentially be targeted and determine whether it is better to knoc...
For example, this method can be applied to study the development and growth of the axons and dendrites of particular cell types or applied to produce sustained over-expression or knockdown of developmental genes in the chick retina using alternative DNA constructs. As shown in our results this ...
2010), additional important information about the virus was acquired using EM and proteomic and RNA knockdown studies in EPC cells (Li et al. 2014b; Ma et al. 2014). These types of studies can provide valuable information on virus replication, which may in turn prove useful for developing ...
including the knockdown resistance gene (kdr) [124,125,126], the acetylcholinesterase gene (ace-1R) mutation [127,128,129,130] and molecular gene markers [131,132,133]. Alternatively, EMS can be used to induce other mutations as has been previously reported for three newAn. quadrimaculatusmu...
Although there is great potential for this system, there are several limitations that need overcome—MBs used for gene therapy are not optimized for DNA binding—US delivery conditions must allow for full perfusion of the target tissue prior to destruction and insonify MBs way that transduces targe...