Many improvements are still necessary before measuring the real value of PCR in microbiological diagnosis.H AgutImmuno-analyse & Biologie SpécialiséeHAgut. (1991) La technique d'amplification génique (PCR) : principle et applications en microbiologie. Immuno-analyse & Biologie Spécialisée 6 , ...
Premier signalement d'une reproduction effective du mérou brun, Epinephelus marginatus, dans la Réserve marine de Cerbère-Banyuls (France) First record of effective reproduction of the dusky grouper, Epinephelus marginatus (Lowe, 1834), in the Cerbere- Banyuls (France) marine protected area. ...
Real-time PCR is becoming a common tool for detecting and quantifying expression profiling of selected genes. Cytokines mRNA quantification is widely used in immunological research to dissect the early steps of immune responses or pathophysiological pathways. It is also growing to be of clinical releva...
This work is supported by the DRUGS4COVID++ project, funded by Ayudas Fundación BBVA a equipos de investigación científica SARS-CoV-2 y COVID-19; and by the Predoctoral Grant (PIPF-2022/COM-25947) of the Consejería de Educación, Ciencia y Universidades de la Comunidad de Madrid, ...
De-Ling Ma Contributions CYZ conceived, designed the study. CCZ performed on nucleic acid extraction, PCR amplification and sequencing. CYZ analyzed the data and interpreted the results. CYZ drafted the manuscript, FXM, YGW, DLM and CCZ jointly revised the manuscript. FXM, CCZ, XPS and JW gav...
17. Stenhouse SA, Plernsub S, Yanola J, Lumjuan N, Dantrakool A, Choochote W, et al. Detection of the V1016G mutation in the voltage-gated sodium channel gene of Aedes aegypti (Diptera: Culicidae) by allele-specific PCR assay, and its distribution and effect on deltamethrin ...
Long PCR improves Wolbachia DNA amplification: wsp sequences found in 76% of sixty-three arthropod species. Insect Molecular Biology 9: 393–405. Article CAS PubMed Google Scholar LaChance, L. E. 1967. The induction of dominant lethal mutations by ionizing radiation and chemicals as related ...
Le facteur de pcription drib est l 'une des séquences de résidus d' acides aminés suivantes: 1) seqid no 1 dans la table de séquences; 2) des protéines qui modulent la résistance des plantes à l 'inversion par substitution, absence ou ajout d' un ou de 10 résidus d 'aci...
schellenbergii, a Real-Time PCR analysis was performed to assess if egg masses treatment enhanced the presence of S. marcescens. RNA was extracted from second instar nymphs derived from control and treated egg masses with the ‘SV Total RNA Isolation System’ (Promega, WI, USA), according to...
This process uses the 5' to 3' nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification assay....