Elson CO, Cong Y, McCracken VJ, Dimmitt RA, Lorenz RG, Weaver CT: Experimetnal models of inflammatory bowel disease reveal innate, adaptive, and regulatory mechanisms of host dialogue with the microbiota. Immunol Rev. 2005, 206: 260-276. 10.1111/j.0105-2896.2005.00291.x. Article PubMed ...
The promoter regions of human APOB gene (-155 to +131 bp), MTTP gene (-165 to +30 bp) and LDLR gene (-1100 to +187 bp) were cloned and recombined into the KpnI/HindIII sites of the pGL3-basic luciferase vector (Promega, Madison, WI). The pGL3-PPRE reporter (PPRE sequence: TCGA...
As expected, the highest cell density was observed in tea bag after infusion at room temperature (9.6 ± 0.3 Log CFU/tea bag). Survival gradually decreased using increasing temperatures of infusion. The cell density decreased from 9.5 ± 0.0 Log CFU/tea bag (75 °C) to 8.5 ± 0.2 Log ...
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room while the wood-look flooring creates a warm tone. the kitchen features modern appliances, an is land, and a pantry for extra storage. the master bedroom offers a walk-in closet and an en suite bathroom with a separate tub and shower. a covered patio looks out onto the large fenced...
FTIR analysis of the MTGase-induced GETG was performed by using a Nicolet 6700 FTIR spectrometer (Thermo Nicolet Co, Ltd., Madison, WI, USA). 1 mL of each sample was taken directly as measured on the attenuated total reflectance (ATR) accessory. The samples were measured by determining the...
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The bacterial 16S rRNA genes of the small intestinal samples were amplified with the primers ENV1 and ENV2. The cloning was performed using the pGEM-T vector system II (Promega, Madison, USA) as previously described [33]. Randomly selected clones were harvested and stored in freezing media ...
The cDNA fragment encoding a sesame caleosin of 245 amino acid residues (accession number AF109921) was constructed in the nonfusion expression vector pET29a (Novagen, Madison, WI, USA), using an NdeI site at the initial methionine position and a XhoI site in the polylinker of the vector [...