Design of candidate aptamers for TDP-43 using catRAPID Our starting point for the catRAPID predictions was a list of more than 3 million RNA fragments identified by iCLIP to bind TDP-4316. From these, we selected contiguous sequences enriched over the experimental control and characterized for ha...
Cells from candidate rafts were assigned a unique barcode by targeted PCR of the sgRNA insert and pooled for NGS sequencing after gel extraction and cleanup (Figures 1A and S1A–S1C). For reference, we picked a representative library containing the six pooled rafts which we ran on the ...