AB_10900242 (BioLegend Cat. No. 109231) Antigen Details Structure Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit Distribution Majority of T cells and thymocytes (correlated to differentiation) Function Antigen recognition, T cell activation ...
4)敲除野生型tcrbeta链的验证 对数生长期的正常人t细胞在体外和步骤3)中包含grna/crispr/cas9基因的慢病毒于30℃离心,共培养12小时后,换细胞培养液。48-72小时后,用流式抗体(anti-tcrbeta,biolegend,cat#109215)进行染色,检测正常人t细胞中野生型tcrbeta链的表达。 检测正常人t细胞表面的tcrbeta链的表达流式抗体...
品牌:BioLegend 产地:进口 生物素 抗小鼠 IgD 抗体价格:￥1300/瓶产品详情:Biotin anti-mouse IgD Antibody详细如下: 405733 Biotin anti-mous... 包装规格:50 ug 用途:科研研究 用途:科研研究 货号:405733 品牌:BioLegend 产地:进口 抗体相关产品报价更多> ...
AB_10933263 (BioLegend Cat. No. 109229) AB_2562562 (BioLegend Cat. No. 109230) Antigen Details Structure Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit Distribution Majority of T cells and thymocytes (correlated to differentiation) ...
AB_2650851 (BioLegend Cat. No. 306736) Antigen Details Structure Ig superfamily, with CD3 forms CD3/TCR complex Distribution T cells, thymocytes Function Antigen recognition, T cell activation Ligand/Receptor Peptide bound to MHC Cell Type
Flow cytometry analysis. The following mAbs recognizing the indicated antigens were used: CD4 (IM2636U, 1:100, Beckman Coulter), CD8 (IM2638U, 1:100, Beckman Coulter); NGFR (345104, 1:40, BioLegend); CD3 (300440, 1:100, BioLegend) and Biotin (409004, 1:100, BioLegend). ...
Flow cytometry was carried out using distinct fluorochrome-conjugated mAb that recognize human CD4, CD8, CD56 (Beckman Coulter), CD45RA, CD197 (BioLegend), human TRBV12-3/TRBV12-4 (Beckman Coulter), and mouse CD4 and CD8 (eBioscience). These mAbs were labeled with Alexa Fluor 488, phy...
(Miltenyi, 130096495). Isolated CD8+T cells were validated by staining with anti-CD8 antibody (3B5), Pacific Blue (Clone 3B5, ThermoFisher, MHCD0828, Lot 2311799) and FITC anti-human CD3 (Clone UCHT1, Biolegend, 300406, Lot B279208). Isolated CD8+T cells were used immediately or ...
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(Biolegend) and a Biolegend MojoSort Magnet, (experiment in Fig.4) or using CD20 MicroBeads (Miltenyi) and an AutoMACS according to the manufacturer’s instructions. A total of 400,000 B cells were activated by seeding 100,000 irradiated hCD40L-expressing L cells (ATCC CRL-2648) in a ...