Lin P, Thornton A, Zhao X, Pan Z, Komazaki S, Brotto M, Takeshima H, Ma J.MG53 nucleates assembly of cell membrane repair machinery.Nat Cell Biol. 2009 Jan;11(1):56-64. doi: 10.1038/ncb1812IF: 21.3 Q1 . Epub 2008 Nov 30. PMID: 19043407; PMCID: PMC2990407. ...
Phospho-TBK1/NAK (Ser172) Rabbit Polyclonal Antibody -20ºC保存,Western一抗稀释液-20ºC或4ºC保存,一年有效。Western一抗稀释液优先推荐4ºC保存,长期不使用可以考虑-20ºC保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。 注意事项: ...
货号:N2402-At488-S 品牌:SYSY Rabbit anti-GFP-Tag Rabbit anti-Polyclonal Antibody 价格: 价格电议 产品详情:温馨提示:优宁维所有产品和服务仅用于科学研究,不用于临床应用及其他用途提供产品和服务(也不为任何个人提供产品和服务)! ... 纯度:见优宁维官网% 包装规格:见优宁维官网 用途:详见说明书 用途:详见...
此外,作者采用了miR-21或let-7 miRISC荧光素酶报告基因实验,发现AGO2显著增强了对荧光素酶报告基因活性的抑制作用,TBK1-WT能显著增强这种抑制作用,TBK1-S172A则不能(图1I),并进一步证实这一点,得到了类似的结果,GFP表达水平受到AGO2的抑制,TBK1-WT的作用更为明显,TBK1-S172A的作用则不明显(图1J). 为了研究...
the decreased activity of the mTOR signaling pathway was not further attenuated by MARCH1 overexpression in TBK1-deficient cells (Figs.2J and S2J). The rescue of shRNA-resistant MARCH1 plasmids in MARCH1-deficient cells suppressed the activation of p-TBK1 S172 (Figs.2K and S2K). In additi...
TANK-binding kinase 1 (TBK1) is a key kinase in regulating antiviral innate immune responses. While the oligomerization of TBK1 is critical for its full activation, the molecular mechanism of how TBK1 forms oligomers remains unclear. Here, we show that p
(A–D) MEFs were treated with 10 ng/mL mTNF-α, +/− Nec-1 s (10 μM). Cell death (A and C) and caspase-8 activity (B) were measured by SytoxGreen positivity and Caspase-Glo 8 assay, respectively. The levels of p-RIPK1(S166) and CC3 were determined by immunoblotting (D...
and unprocessed blots are provided for Figs.1–7and Extended Data Figs.1–6. The mass spectrometry data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org/cgi/GetDataset) through the MASSIVE repository (MSV000084505) under the accession codePXD016025. ...
While depolarization of OPTN−/− cells reconstituted with OPTNWT led to ∼16-fold increase in the ratio of p-TBK1S172/TBK1, the OPTND474N mutant displayed only a 3-fold increase (Figure 3B). The extent of TOMM20 ubiquitylation was equivalent in both OPTNWT and OPTND474N-expressing...
Chemicals, Peptides, and Recombinant Proteins Poly(I:C) Sigma-Aldrich Cat#P1530 Puromycin GIBCO Cat#A1113803 anti-Flag (M2) beads Sigma-Aldrich Cat#M8823 Protein agarose A/G Santa Cruz Biotechnology Cat#20397 His-Trap FF GE Healthcare Life Sciences Cat#17-5138-02 glutathione-Sepharose 4B ...