The reaction was stopped with 2 M sulfuric acid and the plates were read at 450 nm using a POLARstar OMEGA plate reader (BMG Labtech). Preparation of P2 membranes from brain homogenates Mouse brain was isolated and placed in ice-cold homogenization buffer containing the following: 320 ...
Neuron-specific enolase (NSE) IHC (NSE-IHC) protocols in this lab have been previously published13. Statistical analysis. To test the hypothesis that acute CAR treatment after diffuse TBI can reduce inflamma- tory and apoptotic properties at 24 h, associated with CARs ability to ...
This presents us with a conundrum, whereby we cannot fully infer mTBI dysfunction from resting-state changes (because they could be well compensated during natural activity), and we cannot achieve this from targeted tasks (because they may represent “lab” behaviour and are often more difficult ...
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actin immunoblotting (antibody from Cell Signaling Technology Inc., Beverly, MA, USA; 1:200). Antibody-antigen complex was visualized via the Enhanced Chemiluminescence (ECL) method using the Gel Documentation System (BIO-RAD). The density of the bands was calculated using Lab Works software43,...