2ELISPOT操作方法采集8ml患者的血液加入含柠檬酸钠的CPT采血管,分离外周血单个核细胞(PBMC),加入细胞培养液,轻轻重悬细胞。在已包被抗IFN-抗体的微量板的4个孔中,分别加入细胞培养液作为阴性对照,植物血凝素PHA作为阳性对照,结核分枝杆菌特异混合多肽A和B,分别含ESAT-6与CFP-10(Oxford Immunote Ltd., Oxford, ...
The T-SPOT.TB test is also covered in various other clinical lab fee schedules around the world, including the US where it has its own unique CPT code (86481).As well as developing and selling tests in kit form, Oxford Immunotec also runs a testing laboratory business in both the ...
The first is the use of lithium heparinate blood sample collection tubes instead of the CPT? Cell Preparation Tubes with sodium citrate (Vacutainer~R; BD, Franklin Lakes, NJ, USA), as used in the prior study; the other is the use of the T-Cell Xtend~(TM) reagent (Oxford Immunotec) ...
方法 选取85例结核内科医护人员(结核专业人员)为研究组,另选85例健康者为对照组,2组既往均无结核病史、无结核病症状,胸片正常,应用T-SPOT.TB对2组进行外周血结核菌特异γ干扰素(interferon γ,IFN-γ)水平检测,同时进行平行的结核菌素皮肤试验(tuberculin skin test,TST)检测.结果 2组T-SPOT.TB阳性率差异有统计...
采集8ml患者的血液加入含柠檬酸钠的CPT采血管,分离外周血单个核细胞(PBMC),加入细胞培养液,轻轻重悬细胞。在已包被抗IFN-γ抗体的微量板的4个孔中,分别加入细胞培养液作为阴性对照,植物血凝素PHA作为阳性对照,结核分枝杆菌特异混合多肽A和B,分别含ESAT-6与CFP-10(OxfordImmunoteLtd.,Oxford,UK)。然后在每个孔中...
TB test and similar ELISPOT tests have reported sensitivities of 83–97%. [1, 4–10] In addition, compared with the tuberculin skin test, the T-SPOT.TB and similar ELISPOT tests have been shown to correlate more closely with extent of M. tuberculosis exposure in contacts of TB patients. ...
2.ELISPOT操作方法采集8ml患者的血液加入含柠檬酸钠的CPT采血管,分离外周血单个核细胞(PBMC),加入细胞培养液,轻轻重悬细胞。在已包被抗IFN-γ抗体的微量板的4个孔中,分别加入细胞培养液作为阴性对照,植物血凝素PHA作为阳性对照,结核分枝杆菌特异混合多肽A和B,分别含ESAT-6与CFP-10(Oxford Immunote Ltd., Oxford...
T+SPOT+TB技术用于结核的辅助诊断 万方数据
2ELISPOT操作方法采集8ml患者的血液加入含柠檬酸钠的CPT采血管,分离外周血单个核细胞(PBMC),加入细胞培养液,轻轻重悬细胞。在已包被抗IFN-抗体的微量板的4个孔中,分别加入细胞培养液作为阴性对照,植物血凝素PHA作为阳性对照,结核分枝杆菌特异混合多肽A和B,分别含ESAT-6与CFP-10(Oxford Immunote Ltd., Oxford, ...
eta1.Large—scaleevaluationof 与BCG接种以及耐药结核杆菌感染的快速检 enzyme—linkedimmunospotassayandskintestfordiagnosisof Mycobactefium tuberculosis infection against a gradientof 4.总结 综上所述,TSPOTTB技术作为一种 exposureintheGambia.ClinInfectDis,2004,38:966～973 检测结核感染的酶免疫学方法,其敏感...