比较genexpert mtb/rif(简称"xpert")和结核/非结核分枝杆菌pcr荧光探针法(简称"tb-dna")检测肺外结核脓液样本的诊断价值. 方法: 回顾性分析2021年1月至2023年2月在杭州市红十字会医院收治的疑似肺外结核并在病灶部位获取脓液样本的患者...
KEYWORDSTB, GeneXpert, PCR and BAL.Mohammed Soheb Sadath Ansari
Patel VB, Theron G, Lenders L, Matinyena B, Connolly C, Singh R et al (2013) Diagnostic accuracy of quantitative PCR (Xpert MTB/RIF) for tuberculous meningitis in a high burden setting: a prospective study. PLoS Med 10(10):e1001536 ...
Over the years, there has been many methods used to detect tuberculosis such as the Mantoux tuberculin skin test and GeneXpert, the PCR-based technique. In this review, we focused on the detection of interferon-gamma, a tuberculosis cytokine biomarker. Interferon-gamma is conventionally detected ...
B. et al. Diagnostic Accuracy of Quantitative PCR (Xpert MTB/RIF) for Tuberculous Meningitis in a High Burden Setting: A Prospective Study. PLoS Med 10, e1001536 (2013). 42. Nhu, N. T. Q. et al. Evaluation of GeneXpert MTB/RIF for Diagnosis of Tuberculous Meningitis. J. Clin. ...
poured into a single-use disposable cartridge and subsequently loaded onto the GeneXpert module according to the manufacturer’s instructions5. The system automatically interpreted all results from fluorescent signals into the following categories: invalid, if PCR inhibitors were detected with amplification...
NATs coupled with PCR, i.e., nucleic acid amplification testing (NAAT), enables trace or ultra-low detection. In 2010, the WHO endorsed the Xpert MTB/RIF assay (Cepheid, USA) for TB testing [48]. It is the most commonly used test worldwide (>130 countries) [49]. In 2013, the US...
tuberculosis numbering system) of the rpoB gene and is known RRDR. Xpert/MTB RIF is a cartridge-based, automated, hemi-nested real-time PCR system that utilizes five overlapping probes named as Probe A (codons 507–511), Probe B (codons 511–518), Probe C (codons 518–523), Probe D ...
They were then further speciated and probed for isoniazid and rifampicin resistance mutations by PCR, using the Hain Genotype® line probe assay platform (Hain Lifescience GmbH, Nehren, Germany). The Xpert MTB/RIF assay (version G4) was performed at the end of the study on specimens from ...
Diagnostic accuracy of the Xpert MTB/Rif Ultra for tuberculosis adenitis ArticleOpen access13 January 2020 Performance of nested multiplex PCR assay targeting MTP40 and IS6110 gene sequences for the diagnosis of tubercular lymphadenitis Article