2.4. Nucleic acid test Nucleic acid testing (NAT) identifies a species or subspecies of an organism by detecting the genetic make-up, i.e., ribonucleic acid (RNA) or deoxyribonucleic acid (DNA). NATs coupled with PCR, i.e., nucleic acid amplification testing (NAAT), enables trace or ultr...
2.To avoid contamination, it is recommended that the preparation of the organisms be done within a different location than the preparation of the PCR Mix and that different pipettes be used. 3.Care should be taken at all stages of sample DNA collection, extraction and amplification to ensure ...
1. To avoid contamination, it is recommended that sample preparation and PCR Mix preparation be performed within separate locations and with separate pipettes. 2. Care should be taken to label the samples at all stages of DNA collection, extraction and amplification, and to prevent cross-contaminat...
Tuberculosis molecular bacterial load assay (TB-MBLA) is a culture-free test that quantifies the M. tuberculosis 16S rRNA as the marker of viable bacilli using a reverse transcriptase quantitative polymerase chain reaction (RT-PCR). A TB-MBLA assay has been demonstrated to be effective, rapid...
The QuantiFERON-TB Gold test helps diagnose: Latent TB Infection: Presence of TB bacteria without symptoms; the person is not contagious. Active TB Disease: Symptoms are present, and the person can spread TB bacteria to others. Early diagnosis through a "TB testing near me" is crucial for ...
The platform consisting of the Trueprep DNA extraction device and the Truelab micro-PCR machine runs the Truenat MTB Plus assay for detection of Mycobacterium tuberculosis complex (MTBC) and Truenat MTB-RIF Dx for detection of RIF resistance (collectively referred to as the Truenat MTB assays)....
Sample collection Blood was collected for QFT-GIT assays [24] and Human Immunodeficiency Virus (HIV) antibody testing using the Determine™ (Abbot Laboratories, Illinois, USA) HIV 1/2 rapid test. The tubes for the QFT-GIT assay were incubated on site with a portable incubator. HIV testing ...
The qRT-PCR results showed that M.tb infection alone significantly increased mRNA of IL-6, IL-1β, and TNF-α in M.tb-infected RAW264.7 cells (Fig. 2). The siTRIM25 enhanced mRNA expression of IL-6, IL-1β, and TNF-α even more than with the M.tb infection alone (p < 0.01,...
Gene/protein expression was examined by quantitative reverse-transcription–PCR and immunoblotting. The effects of miR-206 and Tbx3 on apoptosis, proliferation, invasion and cancer stem cell population was investigated by cell-death detection, colony formation, 3D-Matrigel and tumorsphere assays. ...
Isolates of Clostridium difficile from 159 hospitalized Danish patients (2005) were analysed by a new 5-plex PCR method targeting the toxin genes tcdA, tcdB, cdtA and cdtB, and 16S rDNA as an internal positive control. Additionally, the toxin-regulating gene tcdC was partially sequenced by a...