#动态#副猪嗜血杆菌荧光PCR检测试剂盒(实时荧光PCR法) 使用说明书 产品详情 公司简介 公司评价 手机查看 手机查看 一键电话致商家 保存条件: 2-8° 数量: 100 保质期: 六个月 用途: 科研实验 产地: 中国 品牌: 雅吉生物 包装规格: 96T 样本: 血清,血浆,组织,尿液脑脊液等 检测方法: 双抗夹...
Routine EBUS-TBNA results for TB PCR, microscopy, culture, cytology and rapid onsite evaluation of cytology (ROSE) where available were reviewed alongside their clinical data. Turnaround times (TAT) from sample collection were measured for each test. Trace readings for Xpert Ultra were analysed ...
2.4. Nucleic acid test Nucleic acid testing (NAT) identifies a species or subspecies of an organism by detecting the genetic make-up, i.e., ribonucleic acid (RNA) or deoxyribonucleic acid (DNA). NATs coupled with PCR, i.e., nucleic acid amplification testing (NAAT), enables trace or ultr...
The coding region (exon2) of the NAT2 gene was amplified by the Polymerase Chain Reaction (PCR) technique using the 96-Well Fast Thermal Cycler (Applied Biosystems, Waltham, Massachusetts, United States). Gene-specific primer set (Inqaba Biotec South Africa), forward 5’GGGATCATGGACATTGAAGCA3...
The assay then amplifies a 192 bp segment of the rpoB gene using a hemi-nested rt-PCR reaction. The assay also contains lyophilized Bacillus globigii spores which serve as an internal sample processing and PCR control. The B. globigii PCR assay is multiplexed with the M. tuberculosis assay....
There- fore, as genomes from the d50 database were used as test samples and tested against genomes in the d10small database, we evaluated the accuracy of strain predictions by assigning a true positive to each strain in a sample if QuantTB predicted the 'correct' relative genome in the d...
If inhibition was observed, the test was repeated with 1 μl of the sample as template. In order to validate the presence of human DNA in the samples an additional PCR was performed for the detection of a 307 bp fragment of human betaglobin. Statistical analysis Data were collected daily ...
tuberculosis complex (MTBC) or non-MTBC by the BD MGIT TBc Identification test (BD Diagnostics, Sparks, MD, USA). They were then further speciated and probed for isoniazid and rifampicin resistance mutations by PCR, using the Hain Genotype® line probe assay platform (Hain Lifescience GmbH, ...
The human β-actin gene was used as an internal standard to correct sample-to-sample variations within a PCR run. Hydrolysis (TaqMan) probes; Probe 47 (Roche GmbH, Cat no: 04688074001), Probe 48 (Roche GmbH, Cat no: 04688082001) and Probe 64 (Roche GmbH, Cat no: 04688635001) from ...
The GeneXpert PCR technique first became available only a few years ago. This allows the early detection of Mycobacterium tuberculosis complex and the detection of multidrug-resistant TB.4 Its sensitivity varies depending on the origin of the sample and the bacterial load.5 Interferon-gamma release...