For Lentivirus production, two 15 cm dishes of HEK293FT cells were seeded at 40% confluence. On the next day, 1 h prior to transfection, the medium was replaced with 13 ml pre-warmed Opti-MEM medium (Thermo Fisher). Transfection was performed using Lipofectamine 2000 and the PLUS ...
H4 cells were seeded at a density of 5000 cells/well in 96-well plates and transfected with 20 ng/well plasmid DNA containing G3BP2 under the CMV promoter (VectorBuilder) and 0.2 μL/well Lipofectamine 2000; Lipofectamine alone served as a negative control. Two days later, 1 μg/ml son...
27.图11是展示了在spr测定中经纯化的抗体与可溶性tau预形成原纤维(pff)的结合的条形图。 28.图12是展示了经纯化的抗体使用细胞荧光能量共振转移(fret)测定抑制或减少tau预形成tau原纤维(pff)诱导的胞内tau聚集体形成的能力的条形图。 29.图13是示出了如在fret测定中使用tau rd p301s fret生物传感器细胞评估的...
In brief, 2–4 μg tau P301L or pCMV plasmids were diluted with Opti-MEM, then added to lipofectamine 3000 regent and P3000 reagent. After 10–15 mins incubation, DNA-lipid complex was added to medium. One day after transfection, 5 μL brain lysate or 20 μL PFF was added into ...
Lipofectamine RNAiMax ThermoFisher Cat # 13778075 Deposited data Bulk RNAseq, cortex, Aldh1l1-Cre+;Bmal1(f/f) vs. Cre-;Bmal1(f/f) controls McKee et al.7 GEO: GSE227063 Wang et al. AD microarray data Wang et al.30 GEO: GSE84422 Seattle Alzheimer Disease Brain Cell Atlas Allen Brain...
a Tau biosensor cells were seeded with aliquots of cortical P-2 from normal controls (NL1, NL2; white), tauopathy cases without plaques (Braak III–IV, plaque 0; T1–5; gray), and AD cases (Braak III–VI, plaque B,C; AD6-8; black), with lipofectamine control (Lipo). Integrated FR...