Download: Download full-size image FIGURE 3. The N terminus of TatA is required and sufficient for the TatA-PspA interaction. A, scheme constructs that resulted in the identification of the N terminus as the region that mediates the interaction with PspA. NT: residues 1–21 of TatA; CT:...
HIV-Tat immunization induces cross-clade neutralizing antibodies and CD4(+) T cell increases in antiretroviral- treated South African volunteers: a randomized phase II clinical trial. Retrovirol 13, 34 (2016).Ensoli, B., Nchabeleng, M., Ensoli, F., Tripiciano, A., Bellino, S., ...
Full membrane translocation as it occurs in cells is likely to depend on additional factors that are not yet included in our reconstitution system. For example, HIV-Tat has been shown to bind to heparan sulfate proteoglycans on cell surfaces (3), a property known to be required for full ...
b (the full sequences of the fusions (GenBank accession numbers: PQ417141 and PQ417142) are offered in the Supplementary Information). A simplified representation of the fusions (without terminal peptides) is shown in Fig.2c, d.
Full size image Figure 5 Quantification of internalized TAT-SA in living HeLa cells. (A) Quantitative analysis of the colocalization between both TAT-SA-A488 and TRITC-labeled transferrin and TRITC-labeled dextran (10 kD) at 15 min post transduction. (B) The relative fluorescence intensity of...
Full size image To simplify comparisons and maximize differences, we have chosen to analyse the models at the time points of maximal protein secretion in each case: for agarase, 60 h (biomass 0.065 gDW/h and protein secretion 6.552·10− 4 mmol/gDW/h), and for α-amylase 24 h (biom...
The Tat protein encoded by HIV-1 is a member of the protein transduction domain family and is rich in basic amino acids. It is widely known that the full-length Tat peptide and the core domain (YGRKKRRQRRR) play important roles in the transduction of heterologous biological macromolecules ...
An abstract is unavailable.doi:10.1097/00042560-199704010-00109Barillari, GFiorelli, VGendelman, RJournal of Acquired Immune Deficiency Syndromes & Human Retrovirology
We evaluated the contribution of UHM–ULM interactions to the association of full-length Tat-SF1 and SF3b1 in human cell extracts (Fig. 7). Plasmids expressing Tat-SF1 variants or N-terminally FLAG-tagged SF3b1 were co-transfected into HEK293T cells. The associated protein complexes were imm...
Download: Download full-size image Introduction Breast cancer is the most common malignant tumor in women, accompanied by the second highest mortality rate, approximately 14% of all female cancer deaths [1]. Currently, clinical therapeutic strategies for breast cancer mainly include surgical resection,...