本抗酒石酸酸性磷酸酶染色试剂盒(Tartrate Resistant Acid Phosphatase Stain Kit, TRAP Stain Kit)以萘酚AS-BI为底物,酸性pH下被ACP水解生成磷酸和萘酚,萘酚与重氮盐偶联生成有色产物,定位于细胞质中。若细胞内ACP含抗酒石酸活性,则呈阳性反应。本品可用于新鲜血涂片/骨髓涂片、细胞爬片、冰冻切片和石蜡切片中的...
产品简介 酸性磷酸酶(acid phosphatase,ACP)分布极广泛,遍布各种组织,主要存在于细胞的溶酶体内,所以常作为溶酶体标志酶。溶酶体外的酸性磷酸酶存在于内质网和胞质内。各种动物中
Technical Bulletin #445 Tartrate Resistant Acid Phosphatase (TRAP) Staining of Osteoclasts on BD BioCoat™ Osteologic™ Discs and MultiTest Slides* Remove medium and wash cells with PBS* Ensure TRAP stain is prewarmed to 37oC* Fix cells with 10% Glutaraldehyde for 15 minutes at 37oC* Wash...
价格: ￥800.00 货号:S0102 规格:3×10ml 品牌:博奥森/Bioss 货期:现货1-2天或期货寻货 购买数量: 产品详情
2004. Fluorescence-based staining for tartrate-resistant acidic phosphatase (TRAP) in osteoclasts combined with other fluorescent dyes and protocols. J Histochem Cytochem 52:411-444.Fluorescence-based staining for tartrate-resistant acid-ic phosphatase(TRAP)in osteoclasts combined with other fluores-cent...
Tartrate-resistant acid phosphatase (TRAP) is a histochemical marker of the osteoclast. It is also characteristic of monohistiocytes, particularly alveolar macrophages, and is associated with diverse pathological conditions, including hairy cell leukemia and AIDS encephalopathy. To study the biology of th...
AIMS: In bone marrow (BM) biopsies, tartrate-resistant acid phosphatase (TRAP) staining represents the gold standard for the characterisation of osteoclasts. TRAP is one of the few enzymes that is histochemically detectable on formalin-fixed paraffin-embedded tissue. This study investigated whether ...
Janckila AJ, Latham MD, Lam KW, Chow KC, Li CY, Yam LT (1992) Heterogeneity of hairy cell tartrate-resistant acid phosphatase. Clin Biochem 25:437–443Janckila AJ, Latham MD, Lam K-W, Chow K-C, Li C-Y, Yam LT. Heterogeneity of hairy cell tartrate-resistant acid phospha- tase....
For identification of semen in stain the specific activity of l -tartrate-inhibitable acid phosphatase (ACP) was determined. With each stain extract, both enzyme activity and protein concentration were determined, and the specific activity (enzyme activity/protein concentration) was calculated. Seminal...