Cytosine base editing enables the installation of specific point mutations without double-strand breaks in DNA and is advantageous for various applications such as gene therapy, but further reduction of off-target risk and development of efficient delive
Cipher block chaining (CBC) is a mode of operation for a block cipher -- one in which a sequence of bits are encrypted as a single unit, or block, with a cipher key applied to the entire block. Continue Reading By Rahul Awati Definition 05 Dec 2024 What are Common Criteria (CC) ...
Reporter assay cloning and transfection CMV-GFP-HP1a (Addgene #17652) plasmid was modified to remove GFP-HP1a sequence using BamHI and HindIII. This backbone was used to clone TALEN or gRNA binding sites in-frame with GFP that was amplified by PCR from the same backbone. Complementary oligo...
These data indicate that KH3 and KH4 can adapt to different AU-rich sequences within the ARE without being limited by a rigid, pre-existing protein-protein interaction (M.F. Garcia-Mayoral et al., submitted). This provides the protein with a flexible recognition unit than can adapt to ...
For the isolation of miRNAs in a plant species, one of the approaches, followed since the beginning inArabidopsisand rice, is based on cloning and sequencing small RNA fractions [7,8]. Once miRNA sequences have accumulated in the public databases, computational strategies have been developed to...
A total of 15 ng of DNA from each sample was combined with the Ampliseq reagents and primer pool for the dysglycaemia according to standard protocol. After this step, the amplicon products were partially digested, and IonExpress Adapters and Barcode sequences were ligated to the library fragme...
MicroRNAs (miRNAs) represent small-single stranded RNA molecules that own the ability to bind to the complementary 3′′-untranslated region (3′′ UTR) of messenger RNA (mRNA) sequences, to post-transcriptionally fine-tune target mRNA gene expression [1,2]. MiRNA biogenesis was proven to be...
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Two homology arms flanking the cleavage sites were inserted into the multiple cloning sites of a modified pHD-DsRed-attP vector (pHD-DsRed-2XattP), which was used as a donor template. The modified pHD-DsRed-attP has a second attP sequence inserted after the loxP. A mixture of the donor...