So there’s a book on my nightstand. The first step to returning to reading on a regular basis was getting to the library and bringing the book home. I am hopeful that actually having a library book on my nightstand which is due back in two weeks will encourage me to open it and r...
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Cells, cultured on glass coverslips, were fixed in 4%paraformaldehydefor 15 min and permeabilized in 0.25% Triton X-100 solution for 30 min at room temperature. Cells were blocked with 1%BSAand incubated with γH2AX antibody (Millipore; 2714539) overnight. Subsequently, the samples were washed ...
Recent Hewlett-Packard Enterprise (HPE) storage acquisitions will play a key role in “reengineering” the company, CEO Meg Whitman said. During HPE’s earnings call Wednesday night, Whitman called ... Continue Reading By Dave Raffo News 01 Jun 2017 New Toshiba SSDs support 64-layer BiCS...
Dyson’s cordless stick vacuums are so light and nimble, they make vacuuming far less of a chore and almost fun. Plus they’re highly versatile – with the tube attachment, it easily reaches under the couch or to the ceiling. Without the tube, it can tidy up tight spaces like a drawer...
Animals were housed in a vivarium with a 12hr light, 12hr dark cycle. Postnatally, experimental animals were kept with their littermates. For timed pregnancies, noon on the day of the vaginal plug was counted as embryonic day 0.5. Transgenic animal models dCAS9-KRAB mouse was a gift from...
After the third centrifugation, the pellets were flash frozen in liquid nitrogen and stored overnight or until use. To prepare extracts, the pellets were thawed on ice in 1.25 ml S30 buffer per gram cell pellet and fully resuspended. The cells were lysed by homogenization on an Avestin ...
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(ISEQ00010, Millipore, USA). After blocking with 5% skim milk, the membranes were incubated overnight at 4 °C with the following primary antibodies: p65 (80979–1-RR, Proteintech, China), p-p65 (bs-0982R, Bioss, China), IκB-α (10268–1-AP, Proteintech, China), p-IκB-α (...