Next generation sequencing (NGS)is the gold standard for analyzing CRISPR editsand is used for 1) unbiased identification and nomination of off-target sites across the genome and 2) accurate evaluation of CRISPR editing events. A combination of assays rooted in this technology allows for ...
Design and Validation of Four New Primers for Next-Generation Sequencing To Target the 18S rRNA Genes of Gastrointestinal Ciliate Protozoa. 喜欢 0 阅读量: 26 作者:Ishaq,Suzanne L.,Wright,André-Denis G.摘要: Four new primers and one published primer were used to PCR amplify hypervariable ...
Anchored multiplex PCR for targeted next-generation sequencing. Nat. Med. 20, 1479–1484 (2014). Article CAS Google Scholar Hoffmann, S. et al. A multi-split mapping algorithm for circular RNA, splicing, trans-splicing and fusion detection. Genome Biol. 15, R34 (2014). Article Google ...
Ready-to-use and custom target enrichment and library preparation reagent kits for precision genomics. Compatible with both DNA and RNA targeted sequencing.
Next-generation sequencing (NGS) preparation of DNA was performed as previously described24. In short, the first PCR was performed to amplify genomic sites of interest with primers containing Illumina forward and reverse adaptor sequences (see Supplementary Note4for oligonucleotides used in this study...
The genomic regions surrounding the off-target site were PCR amplified and then subjected to next-generation sequencing. All primers for the off-target assay are listed in Additional file 1: Table S3. ddddNext-generation sequencing The genomic regions surrounding the on- and off-target sites ...
Target-region capture sequencing is a combination of genomic region enrichment and next generation sequencing which might be used as an efficient way to diagnose various genetic disorders. We aimed to develop a target-region capture sequencing platform to screen 117 selected candidate genes involved in...
Keywords:citrus;bud sports;Target SSR-seq;deep sequencing PDF (1628KB)元数据多维度评价相关文章导出EndNote|Ris|Bibtex收藏本文 本文引用格式 胡冬梅, 江东, 李永平, 彭磊, 李冬云, 朱延松, 杨云光. 利用Target SSR-seq技术鉴定温州蜜柑芽变材料. 中国农业科学, 2021, 54(23): 5083-5096 doi:10.3864/j.iss...
To date, various methods, mostly based on next-generation sequencing (NGS), have been developed to detect the off-target mutations in vivo5,13,14,15. These methods provide genome-wide detection of off-target mutations in an unbiased fashion both, inside and outside the cell. However, the ...
At present, next-generation sequencing (NGS) technology is widely used, and the whole-genome sequences of hundreds of species have been assembled, which has promoted large-scale re-sequencing and produced millions of SNPs for genetic research21. Some SNP genotyping methods combing multiplex PCR and...