Target enrichment sequencing (TES) is a powerful approach to deep-sequencing the exome or genomic regions of interest with great depth. Although successfully and widely adopted in many plant species,...doi:10.1007/978-1-0716-0235-5_10Ze Peng...
We have developed a method, termed target-enrichment of sRNAs (TEsR), that enables targeted sequencing of rare sRNAs and diverse precursor and mature forms of sRNAs not detectable by current standard sRNA sequencing methods. It is based on the amplification of full-length sRNA molecules, ...
Sex determination cascade in insects terminates with the production of sex-specific protein, Doublesex (Dsx). We identified the dsx homolog (Tcdsx) in Tribolium castaneum. The pre-mRNA of Tcdsx is sex-specifically spliced into three female (Tcdsxf1, Tcds
High-throughput sequencing was used to analyze the sequence information in detail. The screen shots of peak enrichment were analyzed by IGV (version 2.12.3). ChIP assay ChIP experiments were performed by following the instructions of a ChIP Analysis Kit (P2078, Beyotime Biotechnology). A Flag ...
CopywriteR allows for extracting uniformly distributed copy number information, can be used without reference, and can be applied to sequencing data obtained from various techniques including chromatin immunoprecipitation and target enrichment on small gene panels. CopywriteR outperforms existing methods and ...
In addition, gene set enrichment analysis (GSEA) was performed to determine KEGG pathways enriched in two metabolic subtypes with ranked genes list (R package “clusterProfiler”).76,84 Corresponding whole-genome sequencing data were also used to explore genomic difference between two metabolic ...
Siglec-6 is a lectin receptor with restricted expression in the placenta, mast cells and memory B-cells. Although Siglec-6 is expressed in patients with chronic lymphocytic leukemia (CLL), its pathophysiological role has not been elucidated. We describe
We applied the method to the GWAS results of the 18 human complex traits from >1.75 million subjects, and identified significant enrichment in rheumatoid arthritis (RA), kidney function, and adult height (P < 0.05/18 = 0.0028, most significant enrichment in RA with P = 1.7...
The graphs show fold enrichment of APP, ADAM10 and BACE1 mRNA levels in the entorhinal (EC) and frontal (FC) cortex from control and AD III-IV/O—C(III-IV) and AD V-VI/B—C(V-VI) samples. Values were determined by Taqman PCR assays, normalized against the levels of HPRT and AARS...
(c) sequencing the DNA of the clone; and (d) mapping the sequence to a eukaryotic chromosome. The present invention is also directed to a polypeptide probe useful in the detection of the expression of a protein capable of binding to a tyrosine-phosphorylated polypeptide portion of a recepto...