TaqMan MicroRNA Assays 适用于多个种属,包括人、小鼠、大鼠、果蝇、线虫和拟南芥。我们将与 Sanger miRBase 数据库同步,持续增加适用于这些种属的 miRNA 测定试剂盒数量。 测定试剂盒的组分 TaqMan MicroRNA 测定试剂盒以两管形式提供:一管含有 RT 引物,另一管含有特定的预先配制的 TaqMan 测定试剂(TaqMan 探针和 ...
•Gold-standard TaqMan qPCR chemistry—TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA families ...
TaqMan™ MicroRNA Assay 市场价¥11268.00 会员价 产品编号4440887 品牌 赛默飞世尔/ThermoFisher 包装 750 RT/750 PCR reactions 规格 750 RT/750 PCR reactions 数量 产品详情 Applied Biosystems TaqMan MicroRNA 检测试剂盒利用高灵敏度和高特异性的Taqman探针法对miRNA进行定量。两步法简单易行,仅需要先使用...
TaqMan Advanced miRNA assays are delivered in a single tube containing the specific pre-formulated TaqMan Assay (TaqMan MGB probe, and forward and reverse primers). Note: TaqMan Advanced miRNA cDNA Synthesis Kit (Cat. No. A28007) and TaqManFast Advanced Master Mix (Cat. No. 4444556) are rec...
Applied Biosystems TaqMan MicroRNA Assay利用高灵敏度和高特异性的Taqman探针法对miRNA进行定量。两步法简单易行,仅需要先使用 miRNA 特异性引物进行逆转录,再利用 TaqMan 探针进行实时荧光定量 PCR。TaqMan MicroRNA Assay特性: ?高度特异性—只对成熟 miRNA 进行定量,而不是前体 ?灵敏—保存有限的样本,只需1–10...
Q52、TaqMan microRNA Assays与TaqMan Advanced miRNA Assays的区别? A: 参见下表: Q53、如何搜索TaqMan MicroRNA Assays的ID 号? A: 建议使用microRNA成熟体序列在Assay Search界面中进行搜索。搜索链接参见条目4 Q54、TaqMan MicroRNA Assays中的反转录引物可以单独订购吗?
由于pri-miRNA转录本还未详尽地定位,所以分析在设计时很接近Sanger miRBase数据库中每个茎环序列,确保对含有目的成熟miRNA的初级转录本进行准确测定。此方法的额外优势是,每个公开的茎环都有一个配对的TaqMan® Pri-miRNA Assay和TaqMan® MicroRNA Assay,让miRNA成熟途径中任一阶段的RNA序列都能独立地定量。
By using TaqMan Pri-miRNA Assays specific to respective pri-miRNA transcripts, transcriptional changes can be directly correlated with changes detected at the mature level for an miRNA of interest. Figure 3. Alignment map viewer. The position of each TaqMan Pri-miRNA Assay is displayed in the ...
A TaqMan MicroRNA Assay has two components: an RT reaction containing a miRNA specific stem-loop reverse-transcription primer and a separate specific TaqMan miRNA Assay. The RT stem-loop primer provides the specificity for the mature miRNA target; it does not detect its precursor. The formation ...
5×TaqMan qPCR Mix 400μL 20×miRNA TaqMan Assay 100μL保存:-20℃避光,避免反复冻融,有效期2年。 配制反应体系按下表加入各成分配制20μL PCR反应体系: 成分 用量 5×TaqMan qPCR Mix 4μL 20×miRNA TaqMan Assay 1μL cDNA模板 1～2.5μL ddH2O 至20μL注意:cDNA模板来自于microRNA反转录产物(...