One unit of the taq DNA polymerase is defined as the amount of enzyme that can incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. Storage Buffer The taq DNA polymerse is supplied in 5 units/µl in 20 mM Tris HCl (pH 8.0), 0.1 mM EDTA, 1 mM ...
Composition Vol Taq 1.0DNA Polymerase1 500 µl(5 U/µl) 10×Reaction Buffer 82 1 ml x 2mM dNTPs 250 µl *1. Wild-type Taq DNA polymerase, prepared by recombinant expression in Escherichia coli, carrying 6 × His tag; This product is in liquid form and contains 50% glycerol and...
3. Product packaging: Product composition 1KU 5KU Storage temperature Hotstart Taq DNA Polymerase (5 U/μl ) 200μl 1ml -20℃ 5×Hotstart Taq Buffer(Mg2+ Plus) 4ml 20ml -20℃ Solution I (10×) 2ml 10ml -20℃ 名称和识别符 质检证书(COA) 相关文档 溶液计算器 图谱搜索服务...
・General composition of PCR reaction mixture (50μl) e2TAK DNA Polymerase 0.5μl 5 × e2TAK Buffer 10μl dNTP Mixture (2.5 mM each) 4μl Template DNA < 100 ng Primer 1 0.2 ～ 0.3μM (final conc.) Primer 2 0.2 ～ 0.3μM (final conc.) Sterilized distilled water up to ...
Discrimination of primer 3'-nucleotide mismatch by Taq DNA polymerase during polymerase chain reaction. The Effect of Single Mismatches on Primer Extension The composition of a 25 uL PCR mixture was 2.5 uL (2 mm) dNTPs, 2.5 uL 10X Taq DNA polymerase buffer, 2.5 uL (25 mm) MgCl2, 1 um ...
extaq说明书
The subject kits, in addition to the above described mutant thermostable DNA polymerase and mutant reverse transcriptase, at least include one of, and usually both of, dNTPs and a buffer composition, where the subject kits may further include additional reagents, including nucleic acids, a thermo...
Invitrogen Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology. ...
• Platinum IITaqHS DNA Polymerase, 1000 μL • 5X Platinum II PCR Buffer, 2 x 12.5 mL • Platinum GC Enhancer, 2 x 12.5 mL Store at -20°C in a non-frost-free freezer. 浏览此商品的顾客同时也查看了 Quant-iT™ PicoGreen™ dsDNA 检测试剂盒 ...
Titanium Taq DNA Polymerase is specially engineered for higher robustness and sensitivity than wild-type Taq, and contains TaqStart® Antibody for hot-start PCR. The polymerase mix is suitable for use in all PCR applications. Separate tubes of optimized PCR buffer (Mg2+ plus) are supplied as ...