Once cooled, strain the liquid into a large container, and serve over ice. Remember to store the leftovers in the refrigerator; it will last for 3-4 days. You’ll know that the drink has gone bad if there are air bubbles floating on top and the liquid is opaque instead of clear!
Soleus muscle, liver were collected and immediately frozen in liquid N2 and stored at -70°C until use for Western blot analysis. Western blot analysis Frozen tissue was homogenized in ice-cold RIPA lysis buffer [150 mM NaCl, 0.5% Triton × 100, 50 mM Tris–HCl (pH 7.4), 25 mM ...