After soaking, bring everything to a boil, and immediately turn the heat down to low. Simmer (with the lid on) for 45 minutes. Add the rock sugar, let it dissolve completely, and turn off the heat to let the li
Soleus muscle, liver were collected and immediately frozen in liquid N2 and stored at -70°C until use for Western blot analysis. Western blot analysis Frozen tissue was homogenized in ice-cold RIPA lysis buffer [150 mM NaCl, 0.5% Triton × 100, 50 mM Tris–HCl (pH 7.4), 25 mM ...
The only thing that should have made a diff is the heat level, but at medium low the liquid should not have evaporated that much. Regardless, when boiling bones for a soup base, feel free to add more water while boiling if the water level goes down too quickly. Reply Lisa says July...