TaKaRa EpiTaq HS (for bisulfite-treated DNA) 说明书
对于T细胞的发育过程来说,针对细胞群体测序,只能提供单个TCR链的频率信息,却不能与细胞进行一一配对,而通过单细胞测序的方式能够进行更为细致的研究。同时,在对特定免疫反应的研究中,通过单细胞TCR测序分析,可以减少群体细胞间异质性信息的干扰。 我们知道一种TCR受体往往在一种类型的免疫反应中发挥作用。因此,对单个T...
FAQs: PCR Polymerases from Takara Bio Contents: PCR Basics Q1 What parameters do I need to consider when designing primers? Q2 What is the optimum amount of template to use? Q3 Which conditions are particularly important to consider when planning your PCR? Recommended Enzymes for Specific ...
For a primer Tm value of 50℃ or less, set in the range of 50 ℃ to 55℃ When amplification product is ≦ 1 kb, set to 10 sec./kb Use at a final concentration of 0.2 µM each Set to 25 to 30 cycles Purify the template DNA URL:http://www.takara-bio.com 7 PrimeSTAR® ...
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BioBrick® 是BioBricks 公司的商标。(http://www.biobricks.org) BioBrick 组装试剂盒包含 –EcoRI-HF –XbaI –SpeI –PstI –10X NEBuffer 2.1 –T4 DNA 连接酶 –10X T4 DNA 连接酶缓冲液 组装两种标准 BioBrick 过程。上游片段用 EcoRI-HF 和 SpeI 消化,下游片段用 XbaI 和 PstI 消化,目的...
两位实验人员分别按照操作说明书,使用 Bio-Rad CFX96 实时荧光定量仪器,用两种荧光定量试剂盒分别对 8 个不同丰度、长度和 GC 含量的靶标(通过不同颜色显示)进行一步法 RT-qPCR 测试。反应结果就以下方面进行评估:扩增效率、低样品量检测能力及无模板扩增值(ΔCq=无模板对照的平均 Cq-最低起始量的平均 Cq)。
Shipping, storage, and handling Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases. Avoid DNA contamination in PCR There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems....
TAKARA BIO INC. Guidelines for Designing Primer: Unknown region S1 5 ' 3 ' 5 ' 3 ' Known region A2 A1 S2 P A. RT-Primer RT-Primer should be 12-15 nucleotides long with a 5' phosphorylated end for the subsequent ligation. It is advisable to phosphorylate the ...
A PCR was carried out in a volume of 100 μl using 5 μl of theArchaeoglobus profundusgenomic DNA solution prepared in Example 1-(1) as a template, and 100 μmol each of RN-F1 and RN-R2 as primers. TaKaRa Ex Taq (Takara Bio) was used as a DNA polymerase for the PCR according...